AMPK Methods and Protocols

14. The plot FSC-H vs. FSC-A is used to discriminate doublets
    from single cells. In this plot, all events falling outside the
    diagonal are considered as a doublet. Doublet cells represent-
    ing less than 5% of total cells can be ignored. If doublet cells
    represent more than 5% of total cells, the formula to calculate
    the number of cells in the population should be adapted as
    follow:

Recovered cellsper1000beads¼

1000 ðÞ 2 nbof events in doublet cell gateþnbof events in single cell gate %studied cell subset

Number of events in bead gate

Acknowledgments

The laboratory is supported by the European Regional Develop-

ment Fund (ERDF), the Walloon Region (Wallonia-Biomed port-

folio, 411132-957270), a Research Concerted Action of the

Communaute ́Franc ̧aise de Belgique, a grant from the Fonds Jean

Brachet, and a research credit from the National Fund for Scientific

Research, FNRS, Belgium, and by the Belgian Program in Inter-

university Poles of Attraction initiated by the Belgian State, Prime

Minister’s office, Science Policy Programming. F.A. is a Research

Associate at the FNRS. T.P. was supported by a FNRS/Te ́le ́vie

fellowship.

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