AMPK Methods and Protocols

(Rick Simeone) #1

  1. In vivo reverse cholesterol transport assay can employ the use
    of wild-type or AMPK-deficient cells. In addition, AMPK acti-
    vators can be used to “prime” cells prior to injection into the
    mice. In this case, it will be critical not only to assess initial
    radioactivity but also to determine cholesterol content in these
    cells.

  2. Cells can also be delivered via subcutaneous injection.

  3. This experiment can test the inherent capacity of cells from
    normal and AMPK-deficient mice, as well as normal and
    AMPK-activator-treated cells. However, there is also the
    potential to use identically treated wild-type cells injected
    into both wild-type and AMPK-deficient mice as recipients.
    This would test whether AMPK could regulate endogenous
    cholesterol acceptors (such as HDL and apoA-I).


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492 Nicholas D. LeBlond and Morgan D. Fullerton

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