Fig. 2Pax7Ki67MyoD labeling and analysis. MuSCs were extracted from total hind limbs as described. (a)
Pax7Ki67MyoD labeling was performed after 48 h of culture in differentiation conditions: Non-treated (top
panel), supplemented with STO-609 (10μm, middle panel) or 2-deoxy-D-glucose (2-DG, 0.5 mM, bottom
panel). Nuclei (Hoescht-Blue), Ki67/MyoD (red), and Pax7 (green) labeling. Full white arrows show Pax7+Ki67/
MyoDquiescent cell; empty white arrows show activated Pax7+Ki67/MyoD+cell; Star shows Pax7Ki67/
MyoD+cell. Scale bar¼ 100 μm. (b) Example of quantification of Pax7+Ki67/MyoD(quiescent), Pax7+Ki67/
MyoD+(activated), Pax7Ki67/MyoD+(committed), Pax7Ki67/MyoD(differentiated) cells
546 Marine Theret et al.