13 Identifying the Heterotrimeric Complex Stoichiometry of AMPK
in Skeletal Muscle by Immunoprecipitation. ............................... 203
Jesper B. Birk and Jørgen F. P. Wojtaszewski
14 Kinase Activity Determination of Specific AMPK Complexes/
Heterotrimers in the Skeletal Muscle ...................................... 215
Jesper B. Birk and Jørgen F. P. Wojtaszewski
15 Determination of Adenine Nucleotide Concentrations in Cells
and Tissues by High-Performance Liquid Chromatography.. ................ 229
Noemı ́Garcı ́a-Tardon and Bruno Guigas
16 Intact Cell Assays to Monitor AMPK and Determine the
Contribution of the AMP-Binding or ADaM Sites to Activation.............. 239
Simon A. Hawley, Fiona A. Fyffe, Fiona M. Russell, Graeme J. Gowans,
and D. Grahame Hardie
17 Cellular Application of Genetically Encoded Sensors
and Impeders of AMPK................................................. 255
Takafumi Miyamoto, Elmer Rho, Allen Kim, and Takanari Inoue
18 Assessing Mitochondrial Bioenergetics by Respirometry in Cells
or Isolated Organelles................................................... 273
Guillaume Vial and Bruno Guigas
19 Study of AMPK-Regulated Metabolic Fluxes in Neurons
Using the Seahorse XFe Analyzer......................................... 289
Claudia Marinangeli, Je ́rome Kluza, Philippe Marchetti,
Luc Bue ́e, and Vale ́rie Vingtdeux
20 Investigating the Role of AMPK in Inflammation........................... 307
Sarah J. Mancini and Ian P. Salt
21 Studying the Role of AMPK in Cardiac Hypertrophy
and Protein Synthesis.................................................... 321
Florence Mailleux, Christophe Beauloye, Jean-Luc Balligand,
Sandrine Horman, and Luc Bertrand
22 Assessment of AMPK-Stimulated Cellular Long-Chain Fatty Acid
and Glucose Uptake..................................................... 343
Joost J. F. P. Luiken, Dietbert Neumann, Jan F. C. Glatz,
Will A. Coumans, Dipanjan Chanda, and Miranda Nabben
23 Measurement of AMPK-Induced Inhibition of Lipid Synthesis Flux
in Cultured Cells.. ..................................................... 363
Marc Foretz and Benoit Viollet
24 Studying the Role of AMPK in Autophagy.. ............................... 373
Sarah Krieg, Bernhard Lu ̈scher, Jo ̈rg Vervoorts, and Marc Dohmen
25 Determining AMPK Activation via the Lysosomal
v-ATPase-Ragulator-AXIN/LKB1 Axis.................................... 393
Chen-Song Zhang, Mengqi Li, Yue Zong, and Sheng-Cai Lin
26 Manipulation and Measurement of AMPK Activity in Pancreatic Islets........ 413
Aida Martinez-Sanchez, Marie-Sophie Nguyen-Tu, Isabelle Leclerc,
and Guy A. Rutter
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