27 Analyzing AMPK Function in the Hypothalamus........................... 433
Patricia Seoane-Collazo and Miguel Lopez
28 Using Ex Vivo Kidney Slices to Study AMPK Effects on
Kidney Proteins......................................................... 449
Renee Rao, Kazuhiro Omi, Roshan Rajani, Hui Li,
and Nuria M. Pastor-Soler
29 A Flow Cytometry-Based Protocol to Measure Lymphocyte Viability
Upon Metabolic Stress.................................................. 465
Se ́bastien Denanglaire, Tiphe`ne Pirnay, Oberdan Leo,
and Fabienne Andris
30 Methods to Evaluate AMPK Regulation of Macrophage
Cholesterol Homeostasis.. .............................................. 477
Nicholas D. LeBlond and Morgan D. Fullerton
31 Modulation of Vascular Function by AMPK: Assessment
of NO Bioavailability and Surrogates of Oxidative Stress..................... 495
Swenja Kro ̈ller-Scho ̈n, Andreas Daiber, and Eberhard Schulz
32 Measurement of Reactive Oxygen Species (ROS) and Mitochondrial
ROS in AMPK Knockout Mice Blood Vessels.............................. 507
Qilong Wang and Ming-Hui Zou
33 Studying the Role of AMPK in Angiogenesis............................... 519
Katrin Spengler, Silke Große, Nderim Kryeziu,
Anne Knierim, and Regine Heller
34 Analysis of Muscle Stem Cell Fate Through Modulation
of AMPK Activity.. ..................................................... 539
Marine Theret, Linda Gsaier, Sabrina Ben Larbi,
Miche`le Weiss-Gayet, and Re ́mi Mounier
35 Evaluating the Role of Host AMPK inLeishmaniaBurden................... 551
Diana Moreira, Je ́roˆme Estaquier, Anabela Cordeiro-da-Silva,
and Ricardo Silvestre
36 Analysis of Transgenerational Phenotypes Following Acute Starvation
in AMPK-DeficientC. elegans............................................ 565
Emilie Demoinet and Richard Roy
37 Humanγ2-AMPK Mutations............................................ 581
Arash Yavari, Dhruv Sarma, and Eduardo B. Sternick
Index.. .................................................................... 621
Contents ix