113fold (10−^1 ), the third yet again diluted tenfold (10−^2 ), and so
on (10−^3 to 10−^6 ).
Spot-calculation: Average of the duplicate counts × 50
(because we plated 20 μL, 1/50 of 1 mL) × dilution factor
(10−^1 to 10−^6 ). That will yield the number CFU/mL.- Primers can be batch diluted to 20 μM and then aliquoted into
separate Eppendorf tubes and frozen at − 20 °C. Then you
only defrost your primers once before usage. - For E. coli RAPD patterns differing by >1 band difference are
considered different clones.
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