156
to place freshly infected embryos in a set volume of E3 (e.g.,
100 μL) within 96-well plates wells. It is also advisable to place
the plates into moist environment (e.g., a box with wet tissue
paper) to prevent evaporation, and therefore keeping the vol-
ume constant throughout the experiment.- The total number of bacteria within an embryo at the end-
point of successful infection is approximately 10^6 CFU per
embryo. - Dissolve low-melting point agarose by heating up the suspen-
sion and cool down to 37 °C before use. - It is important to place the embryos as flat against the glass as
possible to minimize optical distance between the sample and
the microscope lens. Lateral orientation of embryos is pre-
ferred while imaging.
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