viiPreface.............................................................. v
Contributors.............................................................. ix
1 Methods for Measuring the Production of Quorum Sensing
Signal Molecules................................................ 1
Manuel Alcalde-Rico and José Luis Martínez
2 Construction and Use of Staphylococcus aureus Strains to Study
Within-Host Infection Dynamics.................................... 17
Gareth McVicker, Tomasz K. Prajsnar, and Simon J. Foster
3 Method for Detecting and Studying Genome-Wide Mutations
in Single Living Cells in Real Time.................................. 29
Marina Elez, Lydia Robert, and Ivan Matic
4 Detecting Phenotypically Resistant Mycobacterium tuberculosis
Using Wavelength Modulated Raman Spectroscopy..................... 41
Vincent O. Baron, Mingzhou Chen, Simon O. Clark, Ann Williams,
Kishan Dholakia, and Stephen H. Gillespie
5 A Flow Cytometry Method for Assessing M. tuberculosis
Responses to Antibiotics.......................................... 51
Charlotte L. Hendon-Dunn, Stephen R. Thomas, Stephen C. Taylor,
and Joanna Bacon
6 Application of Continuous Culture for Assessing Antibiotic Activity
Against Mycobacterium tuberculosis.................................. 59
Charlotte L. Hendon-Dunn, Saba Anwar, Christopher Burton,
and Joanna Bacon
7 Real-Time Digital Bright Field Technology for Rapid Antibiotic
Susceptibility Testing............................................ 75
Chiara Canali, Erik Spillum, Martin Valvik, Niels Agersnap,
and Tom Olesen
8 Enhanced Methodologies for Detecting Phenotypic
Resistance in Mycobacteria........................................ 85
Robert J.H. Hammond, Vincent O. Baron, Sam Lipworth,
and Stephen H. Gillespie
9 Methods to Determine Mutational Trajectories After Experimental
Evolution of Antibiotic Resistance................................... 95
Douglas L. Huseby and Diarmaid Hughes
10 Selection of ESBL-Producing E. coli in a Mouse Intestinal
Colonization Model............................................. 105
Frederik Boëtius Hertz, Karen Leth Nielsen, and Niels Frimodt-Møller
Contents