Antibiotic Resistance Protocols (Methods in Molecular Biology)

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  1. Ty-Fast cable ties—141 mm (RS components Ltd., Corby, UK).

  2. Tubing connectors, Y shaped for 4–5 mm tubing ID (VWR
    International, Lutterworth, UK).

  3. Tubing connectors, Y shaped for 6–7 mm tubing ID (VWR
    International, Lutterworth, UK).

  4. Tubing connectors, T shaped for 6–7 mm tubing ID (VWR
    International, Lutterworth, UK).

  5. Glass media addition anti grow-back device (D.J. Lee & Co.,
    Ferndown, UK).

  6. In-line connectors (fitting, ¼′′) (Cole-Parmer, Hanwell,
    London, UK).

  7. Acro 37 TF vent device with 0.2 μm PTFE membrane (Pall
    Corporation, Michigan, USA).

  8. Nalgene 2 L, 2125 Heavy-duty wide neck round bottles,
    HDPE (Jencons, Forest Row, UK).

  9. 2 L glass media duran bottle (SLS, Nottingham, UK).

  10. Anglicon magnetic stirrer unit (Brighton Systems, New Haven,
    UK).

  11. Anglicon variable speed pump (Brighton Systems, New Haven,
    UK).

  12. Watson Marlow Bredel 101U/R auto/manual control vari-
    able speed pump (0.06–2 rpm) (Watson Marlow Limited,
    Falmouth, UK).

  13. Watson Marlow Bredel 101U/R auto/manual control vari-
    able speed pump (1.0–32 rpm) (Watson Marlow Limited,
    Falmouth, UK).

  14. Gilson Miniplus 3 peristaltic pump (Gilson, Bedfordshire,
    UK).

  15. 1 L glass media duran bottles (SLS, Nottingham, UK).

  16. Stirring bars, PTFE, wheel—45 mm (VWR International,
    Lutterworth, UK).

  17. Tape heater 5′′ diameter (24v/50w, Brighton Systems, New
    Haven, UK).

  18. Eycoferm fermenter controller (Eycon controller, Eurotherm-
    customized by Brighton Systems, New Haven, UK).

  19. Eycoferm data logging software package (Brighton Systems,
    New Haven, UK).

  20. Glass universal tubes for sampling (20 mm ID) (SLS,
    Nottingham, UK).

  21. Titanium cabinet tubing connectors (NIS, PHE-Porton
    Down).

  22. Digital thermometer (Tempcon Instrumentation limited,
    Ford, UK).


Application of Continuous Culture for Assessing Antibiotic Activity Against...

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