69- Move the tubing through the pumps approximately every
2 weeks in order to maintain elasticity of the tubing and to
reduce the likelihood of splits developing. - Check flow rate (see Note 12) and temperature as required.
4 Notes
- The authors have developed and validated the chemostat system
described in this chapter and recommend the following specific
items as optimal for the performance and safety of the system;
1 L Glass Vessel: DASGIP, Eppendorf, Stevenage, UK. Made to
order 78903189. pH probe (220 mm) plus leads: Mettler
Toledo—gel filled supplied by Brighton Systems, New Haven,
UK, 104054481. Broadley James Dissolved Oxygen (DO)
probe (220 mm) plus leads: Brighton Systems, New Haven,
UK, N1152269. Broadley James membrane kit (for DO probe):
Brighton Systems, New Haven, UK, KC1201. Broadley James
filling solution (DO probe electrolyte): Brighton Systems, New
Haven, UK, AS-3140- 130-0025. DOT signal amplifier:
Brighton Systems, New Haven, UK. Dual-wire PFA sheathed
stainless steel RT probe plus leads (temperature probe): Brighton
Systems, New Haven, UK, Made to order. Portex silicone rub-
ber tubing—6 × 2 mm: SLS, Nottingham, UK, TUB3808.
Sterilin silicone tubing—4 × 1.6 mm: SLS, Nottingham, UK,
TUB7042. Esco silicone tubing—1 × 2 mm: SLS, Nottingham,
UK, TUB7012. Tubing connectors Y shaped for 4–5 mm and
6–7 mm tubing: VWR International, Lutterworth, UK, 229-
3442 and 229-3444. Tubing connectors, T shaped for 6–7 mm
tubing: VWR International, Lutterworth, UK, 229- 3424. Glass
media addition anti grow-back device: D.J. Lee & Co.,
Ferndown, UK, Made to order. In-line connectors (fitting, ¼′′):
Cole-Parmer, Hanwell, London, UK, 06360-90. Anglicon
magnetic stirrer unit: Brighton Systems, New Haven, UK,
MS01. Anglicon variable speed pump: Brighton Systems, New
Haven, UK. Watson Marlow Bredel 101U/R auto/manual
control variable speed pump (0.06–2 rpm): Watson Marlow
Limited, Falmouth, UK, 010.4002.00U. Watson Marlow
Bredel 101U/R auto/manual control variable speed pump
(1.0–32 rpm): Watson Marlow Limited, Falmouth, UK,
010.4202.00U. Gilson Miniplus 3 peristaltic pump: Gilson,
Bedfordshire, UK, F155001, F117800. Tape heater 5′′ diame-
ter (24v/50w): Brighton Systems, New Haven, UK, Made to
order. Eycoferm fermenter controller: Eycon controller,
Eurotherm- customized by Brighton Systems, New Haven,
UK. Eycoferm data logging software package: Brighton Systems,
New Haven, UK, Made to order. Clips, tubing—30 mm: VWR
3.5.3 Occasionally
Application of Continuous Culture for Assessing Antibiotic Activity Against...