Front Matter

Contribution of Fragrances to ASD 181

(a)

(b)

(c)

1 A B C D E

234 5 6 7 8 9

Day 0Day 2Day 4Day 6Day 8

NBC cell line containing

unknown numbers of

progenitor cells

Growth pattern of a single-cell clone from a single cell into a larger cluster of cells from day 0 to day 8

Each of the single-cell clones

were expanded and the

clones were characterized

with monoclonal antibodies

to brain specific antigens

Single cells were seeded into

each well of 96-well plate and

single cells were allowed to grow

until each single-cell clone reach

confluency. The single-cell

clones were then transferred into

larger flask and allowed to grow

until 90% confluency Screened with monoclonal

antibodies

Figure 7.1 (a) Various kinds of neuronal progenitor cells. Each color represents a unique
progenitor cell in weeks 4–8 fetal brain, destined to become one of the compartments of
an adult brain. Elimination of one of the cell types may result in complete or partial absence
of a future compartment of a brain. Depletion of a single cell at an early developmental stage
can result in a cognitive deficit. (b) The process of single‐cell cloning. The neuroblastoma
cell line is cultured in a specialized cell culture flask, and the cells are then trypsinized to
separate each of the cells (each representing a unique fetal brain progenitor cell). The
individual cells are seeded into a 96‐well plate until they grow in a cellular cluster. Each of
the clusters is then transferred into a new culture flask. Each clone is then characterized by
immunostaining (c). (c) The visual microscopic presentation of a single-cell clone that grows
into a large cluster-from day 0 to day 8. (See insert for color representation of this figure.)