408 Canine Sports Medicine and Rehabilitation
and greatest release of anabolic growth factors
(Franklin et al., 2017a) (Figure 16.4). Importantly,
assessment of frozen samples of this particular
PRP showed that the anabolic growth factor
concentrations from the frozen sample were
similar to those obtained with calcium chloride
activation, thus raising the question of whether
frozen and thawed PRP samples might be(D)(A) (C)(B)Figure 16.4 (A) Resultant platelet‐poor plasma, platelet‐rich plasma (PRP), and calcium/thrombin mixture that can be
used to activate the PRP and form a platelet‐rich fibrin gel. (B) Platelet‐rich plasma placed in a sterile glass tube for
intraoperative activation and formation of a platelet‐rich fibrin gel. (C) Injection of bovine thrombin and calcium
chloride into a sterile glass tube of platelet‐rich plasma to form a platelet‐rich fibrin gel. (D) A platelet‐rich fibrin gel is
formed by activating platelet‐rich plasma with thrombin/calcium chloride.