304 K. Mackie
Fig. 2A–C.CB 1 expression in serial sections of aγ-aminobutyric acidergic (GABAergic) terminal synapsing
onto an apical dendrite in cortex. CB 1 receptors (arrowheads) were detected with an antibody directed against
the C terminus of rat CB 1 using pre-embedding immunogold with silver enhancement. The boutons are
forming symmetric synapses (arrows), characteristic of GABAergic axon terminals, onto the apical dendrite of
a cortical pyramidal cell.Scale bar= 0.5 μm. (Original photomicrograph provided by Tamas Freund and Agnes
Bodor)
Fig. 3A–C.Reciprocal expression of CB 1 and FAAH in mouse hippocampus. FAAH was detected using an
antibody raised against the last 200 residues of FAAH, CB 1 receptors were detected by an antibody directed
againstitsCterminus,andneuronalnuclearantigen(NeuN)wasdetectedusingamousemonoclonalantibody
from Chemicon. FAAH is expressed uniformly by pyramidal neurons (Pyr) including the apical dendrites. FAAH
is also expressed in interneurons (open and filled arrows). CB 1 receptors are present in axons investing the
pyramidal cell layer and also some interneuron cell bodies (filled arrows), but not in others (open arrow).
Staining of neurons by NeuN identifies neuronal nuclei in the field.Scale bar= 18 μm. (Figure provided by
Tibor Harkany)