Basic Concepts in Clinical Biochemistry-A Practical Guide.7z

(Chris Devlin) #1

Blood (cont.)
pH, 5, 25, 122
Bradshaw’s test, 36
Brij 35, 46
Bromo cresol green (BCG), 46– 48
Buffers, 21–27, 33, 34, 46, 58, 64, 65, 86, 87,
104, 105, 108, 111, 112, 118, 130, 132,
140 – 142


C
Calcium
clinical significance, 87
distribution, 86
functions, 85
hypercalcemia, 88
hypocalcemia, 88, 159, 167
ionized, 85, 88
protein bound, 85
reference range, 85, 159
Calibration, 9, 10, 12, 104
Capillary blood, 5
Carbonate-bicarbonate buffer, 25, 108, 112
Case studies
calcium & phosphorus impairment, 159
cardiac functions, 167
diabetic ketoacidosis, 158
gout/uric acid, 160
kidney functions, 165– 167
liver functions, 162
protein energy malnutrition, 160
sugar impairment, 157, 158
Cerebrospinalfluid (CSF)
clinical significance, 137
CSF protein analysis, 135– 137
functions, 135
occurrence, 135
reference range, 137
secretion, 135
Cherry Crandell Unit (CCU), 118
Cholesterol
absorption, 93
clinical significance, 95
esterified cholesterol, 93
functions, 93
occurrence, 93
reaction, 94
structure, 93
Cholesterol esterase, 139, 140
Cholesterol oxidase, 139, 140, 142


Chylomicrons, 93, 139, 141– 143
Clinistix, 50– 51
Color reagent, 68, 69, 104
Colorimeter, 17–20, 98
Creatine, 75, 131– 133
Creatine kinase (CK), 131–133, 167
Creatinine
albumin-creatinine ratio, 33
clinical significance, 78
creatinine clearance, 79, 80
metabolism, 76
reference range, 78
synthesis, 75
transport, 75
Cresolphthalein, 86, 87

D
Diacetyl monoxime, 68
Diazo reagent, 98–100, 125
Dipstick, 34
Direct bilirubin, 97–101, 163
Double beam spectrophotometer, 19

E
Enzyme linked immunosorbant assay (ELISA)
antibody, 153– 155
applications, 155
competitive, 154
direct, 155
indirect, 155
principle, 153, 154
sandwich, 153
Equivalent weight, 22, 23
Ethylenediaminetetraacetic acid (EDTA), 6, 7,
81, 94, 107, 131, 132
External quality control, 12

F
Fibrinogen, 5, 6, 43
Flame photometer, 147– 150
Fouchet’s test, 123, 124, 126

G
Globulins, 33, 34, 39, 43, 48, 85, 137, 163
Glomerularfiltration rate (GFR), 71, 79, 80, 92
Glomerular proteinuria, 36, 37

172 Index

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