Basic Concepts in Clinical Biochemistry-A Practical Guide.7z

(Chris Devlin) #1

The sample cuvette used in spectrophotometer is made of quartz instead of glass
cuvette as in colorimeter. Although glass cuvette can be used for wavelength
measurement in visible range, but only quartz cuvette can be used for wavelength
measurement in UV region since glass absorbs UV light. The light source used is
deuterium lamps for the UV measurement, whereas tungsten or halogen lamp is used
for measurement in visible range of electromagnetic spectrum. These days, xenon
arc lamps are also used which produces light of both UV and visible spectrum.


20 5 Photometry: Colorimeter and Spectrophotometer

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