Basic Concepts in Clinical Biochemistry-A Practical Guide.7z

S. no

Volume

of 0.2 M

Tris

taken

Amount of

0.2 M Tris in

used volume

(μmole)

Volume

of 0.2 M

HCl

taken

Amount of

0.2 M HCl in

used volume

(μmole)

Volume of

distilled

water

added (ml)

Theoretical

pH

1. 50 10,000a 5 1000 45 9.1a


2. 50 10,000 10 2000 40 8.8


3. 50 10,000 15 3000 35 8.6


4. 50 10,000 20 4000 30 8.5


5. 50 10,000 25 5000 25 8.4

aTheoretical pH can be calculated as explained for acetate and phosphate buffer.

6.6 Preparation of Common Laboratory Buffers 27