- If the urine sample used is less than 2 ml for the titration, dilute urine 1 in 5 or 1 in
10 with distilled water and include the dilution factor in the calculation, after
doing a rough titration. - A greenish-blue color will reappear ifflask is allowed to stand. This is reoxidation
process of air and may be ignored.
12.8 Calculation
50 mg of glucose will reduce 25 ml of Benedict’s quantitative reagent. Suppose titer
of urine is 10 ml, then 10 ml urine must contain 0.05 g glucose and then amount of
glucose/100 ml urine
¼
50 100
10
¼xmg=dl12.9 Clinical Significance...............................
The clinical significance of glycosuria has been discussed in previous chapter.
12.9 Clinical Significance 55