Table 8.1
Developed RNAi switches.
ReferencesAnet al. [10]
Beiselet al. [11]
Kumar et al.[12]Masu et al. [13]
Beiselet al. [14]
Kumaret al. [15]
Saitoet al. [16]
Kashida et al. [17]
Afonin et al. [18]
Velagapudi et al. [19]
InputSmall molecule (theophylline)Small molecule (theophylline, hypoxanthine)Small molecule (theophylline)RNASmall molecule (theophylline, hypoxanthine)Chemically modified RNAArchaeal protein (L7Ae)Human protein (U1A, NFκB)Chemically modified DNASmall molecule libraryDose~10mM~5mM~3mM~500nM~5mM~500nMPlasmid expressionPlasmid expression~500nM~40μMBased RNAshRNAshRNAPri‐miRNAsiRNAPri‐miRNAPri‐miRNA+
siRNAshRNAshRNAsiRNAPre‐miRNAInhibiting machineryDicerDicerDroshaDicerDroshaDrosha+
DicerDicerDicerDicerDroshaSwitchON→OFFON→OFFOFF→ONOFF→ONON→OFFOFF→ONON→OFFON→OFFOFF→ONON→OFFTarget geneEGFP, DsRedEGFPEGFPLuciferaseEGFPEGFP, DsRedEGFP, antiapoptotic gene (Bcl‐xL)EGFPEGFP, HIV‐1 Gag‐pol glycoproteinProapoptotic gene (FOXO1)Cell typeHEK 293HEK 293‐EGFPHEK 293HeLaHEK293(T), HeLa, MDA‐MB‐231, HEK293T‐EGFPHEK 293HeLa, HeLa‐EGFPHEK293FTHeLa, MDA‐MB231, MDA‐MB231‐EGFPMCF7