344 16 Applying Advanced DNA Assembly Methods to Generate Pathway Libraries
Pathway libraries A collection of mutant enzymatic pathways wherein multiple
components (RBS, promoters, enzymes) within the pathway have simultane-
ously been mutated
Directed evolution An evolutionary process for engineering biological systems
that mimics Darwinian evolution in vitro and in vivo: rounds of random muta-
tions are incorporated into the DNA sequence and selected for improved phe-
notype in an iterative fashion
DNA assembly The process to conjoin several DNA fragments to create a large
DNA moleculeReferences
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