404 Index
Chinese hamster ovary (CHO) 314
circular polymerase extension cloning
(CPEC) 334
comparative genomics 56, 64
context‐dependent assembly
(CoDA) 37
controlled splicing in S. cerevisiae
alternative splicing 145
function of 147
regulated splicing 146
core promoter 110
CPEC see circular polymerase extension
cloning (CPEC)
CRISPR/Cas9 system 63
CRISPR‐enabled trackable genome
engineering (CREATE) 27d
De novo organelle 295
directed evolution 341
DNA assembly methods
BioBrick system 334
circular polymerase extension
cloning 334
Golden Gate 334
homologous recombination 333
pathway libraries 335
polymerase extension 333
DNA‐binding proteins
dimers or tetramers 250
TAL‐DNA binding domains 249
zinc‐finger domains 248–249
DNA guided assembly 255
DNA‐guided programming
applications 253–254
DNA program
number of scaffold repeats 252
spacers 250
spatial position of biosynthetic
enzymes 251
target site arrangement 253
DNA scaffold
applications of 253–254
binding proteins 247
biosynthetic applications 242
biosynthetic enzymes 239–240
program 250vs. protein and RNA 241
DNA sequencing 3, 5–7
DNA synthesis 3, 8
productivity of 4
DNA‐target site 241
double‐stranded DNA (dsDNA) 3, 10e
E. coli genome 51
E. coli system 311
encapsulins 291–292
ePathBrick system 338
eukaryotic platforms 312
European Academies Science Advisory
Council 390
exons 132, 134f
functional peptides
binders 220
labeling‐enzymes 221
protease cleavage sites 222
reactive peptides 223
fusion protein 245g
gender equality 391
gene design
alternative splicing in mammals 137
controlled splicing in S. cerevisiae 145
elements of mammalian and budding
yeast pre‐mRNAs 133
influence of introns 152
nuclear pre‐mRNA splicing in
mammals 132
self splicing introns 136
splicing and synthetic biology 150
splicing in yeast 135
splicing regulation by
riboswitches 147, 149
split genes 131
tRNA splicing 137
gene essentiality 55
gene synthesis 9
genetic circuit optimization 321
genetic diversity‐generating factors 59
genetic engineering 386