Microfluidics for Biologists Fundamentals and Applications

2 Centrifugal Microfluidics One of the main challenges for improving the efficiency and reliability of the resulting diagnostic ...

Fig. 5.1 Agraphical representation of the direction each force acts in for a give direction and constant spin rate (Centrifugal ...

v¼ ρ 32 η rd^2 ω^2 ð 5 : 4 Þ and the volumetric flow rate Q¼~vA¼ πρ 128 η rd^4 ω^2 ð 5 : 5 Þ with the cross sectional area of th ...

predetermined volume of liquid in the initial chamber. The liquid in this chamber then can be released by opening the valve, val ...

Determined by the Molecular Diffusion CoefficientDwhich can range consid- erably between bio-molecules; for small molecules, the ...

immunoassay on-disk is the Enzyme linked Immunosorbent Assay or ELISA, this technique requires a solid support with bound captur ...

3.1.2 Siphon Valves The structure of a siphon valve typically utilizes hydrophilic microchannels to ensure capillary action domi ...

rotation is increased the fluid will move around the siphon to fill the next chamber. A number of these siphon valves can be pla ...

3.1.3 Centrifugo-Pneumatic Valving This type of Lab-on-a-Disc platforms create structures between layers of different materials ...

unit operations downstream, this is known as the Load Film (LF). The second DF is located within a second chamber which is conne ...

3.2.2 Electromechanical Pumping Clime et al. created a microfluidic disk with integrated microelectronics responsible for on-dis ...

camera and strobe. Thus, each image of the disc is acquired at the same orientation; a video generated from these still frames s ...

Fig. 5.8Spin stand Operation (a) shows an image of a typical spins stand. The motor is mounted vertically and the camera is moun ...

5.1.2 Positional Triggering If the motor generates just one digital pulse, at the home position, per rotation then the disc can ...

5.2 Application Example: Automation of a Direct Bilirubin Test 5.2.1 Direct Bilirubin Direct bilirubin is a measurement of bilir ...

purified, 20μL is removed and added to each of the two test reagents. The results can then be obtained by measuring the absorban ...

Fig. 5.11 Schematic Diagram representing the disk architecture and liquid handling protocol 132 B. Henderson et al. http://www.e ...

6 Manufacturing Protocol 6.1 Designing the Lab-on-a-Disk The designs used in this protocol were created in the commercial drawin ...

The microfluidic discs are created using layers of poly(methyl methacrylate) (PMMA) bonded together by layers of pressure sensit ...

6.2 Cutting PSA Sheets of pressure sensitive adhesive are cut using a Graphtec automated knife cutter. This device is controlled ...