then rolled at room temperature in the hot roll laminator, ensuring that the disk is
void of trapped air bubbles (Fig.5.22).
This procedure is held throughout the assembly process. This Lab-on-a-disk
platform is layered as follows:
7.1 Vents (PMMA)
Holes are cut from the a PMMA layer in specific locations to allow air to enter/leave
the chamber as fluid enters/leaves to ensure the chamber remains at atmospheric
pressure, meaning there is no resistance to the movement of fluid. This layer can
also act as a pneumatic seal to create pressurised chambers which restrict the flow of
fluid like in the case of dissolvable film valves (Fig.5.23).
Fig. 5.22 Hot Roll Laminator used to activate PSA
Fig. 5.21 A PSA layer (Left) is bonded to a PMMA layer using a pressurised roller
138 B. Henderson et al.