Table 4.2.
(Continued
)
Orchid species, cultivars,or hybrids
Explant type
Cryopreservationtechnique
z
Survival (%) ofexplants post-cryopreservation
References
Cattleya intermedia,
Encyclia pygmaea, E.odoratissima, Grobyasp
., Oncidium
flexuosum, O. pumilum
Seed
Temperature (5
◦C)
90% (24 months)
Pardo-Alvarez and Ferreira
(2006)
Oncidium bifolium
Seed
Encapsulation–
dehydration
80% of the
protocormssurvived after7hofdehydration;11.3% ofprotocormsresulted inplants
Flachsland et al. (2006)
Dendrobium
unregistered
hybrids (“Sena Red” and“WRL”)
Seed
Dehydration–vitrification
(PVS2)
50% higher than
the control
Vendrame et al. (2007b)
Vanda coerulea
Seed
Vitrification
0%
Thammasiri and Soamkul (2007)
Dendrobium chrysotoxum
Seed
Vitrification 50 min PVS2
(25
◦C
±
◦ 2
C)
99%
Thammasiri (2008)
Dendrobium cruentum
Seed
Vitrification 30 min PVS2
(25
◦C
±
◦ 2
C)
32%
Thammasiri (2008)
Dendrobium draconis
Seed
Vitrification 30 min PVS2
(25
◦C
±
◦ 2
C)
95%
Thammasiri (2008)