Horticultural Reviews, Volume 44

(Marcin) #1

Table 4.2.


(Continued


)


Orchid species, cultivars,or hybrids

Explant type

Cryopreservationtechnique

z

Survival (%) ofexplants post-cryopreservation

References

Cattleya intermedia,

Encyclia pygmaea, E.odoratissima, Grobyasp

., Oncidium
flexuosum, O. pumilum

Seed

Temperature (5

◦C)

90% (24 months)

Pardo-Alvarez and Ferreira

(2006)

Oncidium bifolium

Seed

Encapsulation–

dehydration

80% of the

protocormssurvived after7hofdehydration;11.3% ofprotocormsresulted inplants

Flachsland et al. (2006)

Dendrobium

unregistered

hybrids (“Sena Red” and“WRL”)

Seed

Dehydration–vitrification

(PVS2)

50% higher than

the control

Vendrame et al. (2007b)

Vanda coerulea

Seed

Vitrification

0%

Thammasiri and Soamkul (2007)

Dendrobium chrysotoxum

Seed

Vitrification 50 min PVS2

(25

◦C

±

◦ 2
C)

99%

Thammasiri (2008)

Dendrobium cruentum

Seed

Vitrification 30 min PVS2

(25

◦C

±

◦ 2
C)

32%

Thammasiri (2008)

Dendrobium draconis

Seed

Vitrification 30 min PVS2

(25

◦C

±

◦ 2
C)

95%

Thammasiri (2008)
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