Table 1
Quantitative copy numbers of single-cell proteins
Technique Key results
Flow cytometry Thy-1, Lyt-1, Lyt-2, and Lyt-3 of single positive T cells were quantified within the
range of 10^5 –10^6 per cell [9]
Flow cytometry Fc receptors of positive lymphocytes (5–25%) were quantified as 4.2 104 per cell
[10]
Flow cytometry Fc receptors of small and large monocytes were quantified as 3.30.6 105 and
10 1 105 per cell, respectively [11]
Flow cytometry Surface antigens of TI01, RFTI1, OKT3, OKT4, OKT8, OKT6, and 3AI of single
cells of RPMI 8402, MOLT-4, CEM, and HSB-2 were quantified in the range of
103 –10^5 copy numbers per cell [12]
Flow cytometry Fc receptors of monocytes were quantified as 1.750.34 104 per cell [13]
Flow cytometry PI-bound proteins of blood cells were quantified within the range of 10^4 and 10^5
per cells [14]
Flow cytometry CD11a of CD8+ lymphocytes was quantified, locating the value of 4.3 103 per
cell as the cutoff point between high and low expressions [15]
Flow cytometry CD10 of normal lymphoid precursors was quantified as 3–12.5l0^3 per cell with
an upper limit of 5 104 per cell. The median CD10 in acute B-lineage acute
lymphoblastic leukemia were variable and ranged from undetectable to very high
values (>1.8l0^5 )[16]
Flow cytometry Normal TdT+precursors have a significantly higher number of TdT (> 100 103 )
and a lower number of CD10 (< 50 103 ) and CD19 (< 10 103 ) molecules
per cell than B-lineage ALL blasts (<l00,>50,> 10 103 molecules per cell,
respectively) [17]
Flow cytometry For T cells, CD2, CD3, CD4, CD5, CD7, and CD8 were quantified as 247,
57 7, 4714, 5011, 287, and 14529 ( 103 /cell), while for B cells,
CD19, CD20, CD21, CD22, CD24, and CD37 were quantified as 273,
149 10, 122, 172, 392, and 9926 ( 103 /cell) [18]
Flow cytometry CD3, CD4, CD8, CD16, and CD19 of lymphocytes and CD4 of monocytes were
quantified as 134,34,945,905, 54,17,446,106, 268,868–190,622, 38,307,436,
25,252–11,689, and 9246–3094 per cell, respectively [19]
Flow cytometry For subsets of lymphocytes, CD2, CD3, CD4, CD5, CD7, and CD8 were
quantified in the ranges of 54.78–59.38, 20.71–35.56, 34.41–54.61,
81.85–94.33, 26.53–65.05, and 30.46–130.96 ( 103 per cell) [20]
Flow cytometry CD3 molecules were quantified as 124–25 (normal T cells), 30–21 (T lymphocytes
from healthy donors), 38–31 (adult T-cell leukemia/lymphoma), 92–47
(T-prolymphocytic leukemia), and 95–21 (granular lymphocyte leukemia)
( 103 per cell); CD7 molecules were quantified as 20–3 (normal T cells), 35–7
(T lymphocytes from healthy donors), 13–3 (adult T-cell leukemia/lymphoma),
29–12 (T-prolymphocytic leukemia), and 12–3 (granular lymphocyte leukemia)
( 103 per cell) [21]
(continued)296 Beiyuan Fan et al.