Table 1
(continued)
Technique Key results
Flow cytometry CD19 molecules were quantified as 22 (normal B cells), 13 (chronic lymphocytic
leukemia), 16 (B prolymphocytic leukemia), 15 (splenic lymphoma with villous
lymphocytes), 10 (mantle cell lymphoma), and 38 (hairy cell leukemia) ( 103
per cell); CD20 molecules were quantified as 22 (normal B cells), 13 (chronic
lymphocytic leukemia), 16 (B prolymphocytic leukemia), 15 (splenic lymphoma
with villous lymphocytes), 10 (mantle cell lymphoma), and 38 (hairy cell
leukemia) ( 103 per cell) [22]
Flow cytometry CD19 molecules were quantified as 131 (normal B cells), 44 (chronic
lymphocytic leukemia), 87 (B-cell prolymphocytic leukemia), 44 (mantle
cell lymphoma), and 45 splenic lymphoma with villous lymphocytes ( 103 per
cell); CD5 molecules were quantified as 112 (normal B cells), 31 17
(chronic lymphocytic leukemia), 1610 (B-cell prolymphocytic leukemia),
24 14 (mantle cell lymphoma), and 189 splenic lymphoma with villous
lymphocytes ( 103 per cell); CD79b molecules were quantified as 43 8
(normal B cells), 23 (chronic lymphocytic leukemia), 3718 (B-cell
prolymphocytic leukemia), 1716 (mantle cell lymphoma), and 1213 splenic
lymphoma with villous lymphocytes ( 103 per cell) [23]
Flow cytometry CD19, CD20, CD22, CD23, CD79b, and CD5 of typical chronic lymphocytic
leukemias and atypical chronic lymphocytic leukemias were quantified within the
ranges of 3844–38,347 vs. 2022–23,300, 1900–109,600 vs. 4000–70,640,
1689–20,941 vs. 2790–28,679, 1736–22,342 vs. 2011–3768, 1721–5087
vs. 2043–10,840, and 1790–59,258 vs. 2620–24,440, respectively [24]
Flow cytometry CD23 molecules were quantified in the range of 0.6–167.3 (chronic lymphocytic
leukemia), 1.1–85.2 (chronic lymphocytic leukemia with prolymphocytes),
0.6–42.7 (B-cell lymphoma), and 0.8–3.6 (control) ( 103 per cell) [25]
Flow cytometry CD10, TdT, CD34, CD19, and CD79a of normal and leukemic bone marrow
precursors were quantified in the ranges of 8.1–23.4 vs. 17.1–211.7, 90.7–314.8
vs. 20.8–582, 99.7–280.8 vs. 1.9–423.5, 20.6–33.2 vs. 8.2–85.8, and 5.9–7.8
vs. 1.7–37.5 ( 103 /cell) [26]
Flow cytometry Estrogen receptors were quantified as 92131572 (MDA-MB-231),
5912 1768 (SKBr3), 57,76512,998 (T47-D), 104,00016,683
(MCF-7), and 6267–1,390,000 (clinical breast tumor samples) (per cell) [27]
Flow cytometry CD58 molecules in regenerating, normal, and leukemic bone marrows were
quantified as 1649837, 1705669, and 11,2457672 (per cell) [28]
Flow cytometry CD11b molecules of single polymorphonuclear cells were quantified as 79,440 of
Aspergillussepticemia postrenal transplantation, 80,090 of septicemia
Streptococcus bovispostvalvuloplasty infection, 37,330 of meningoencephalitis to
Herpesviridae, 36,060 of aortic endocarditisStreptococcus agalactiae, 53,430 of
septicemiaStreptococcus mitis, 36,050 of pneumopathy toStaphylococcus aureus,
37,790 of pneumoniaChlamydia psittaci, 14,300 of HIV infection, 34,090 of
pneumoniaPseudomonas aeruginosa, 42,550 of acute pyelonephritis toProteus
mirabilis, 45,680 of endocarditis toStreptococcus viridans, 85,360 of peritonitis
secondary to appendicitis, 16,210 of hepatitis B infection, 57,920 of acute
pancreatitis, and 69,540 of septicemia toStaphylococcus aureus(per cell) [29]
(continued)Single-Cell Protein Assays: A Review 297