Computational Systems Biology Methods and Protocols.7z

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Table 1
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Technique Key results
Flow cytometry CD51 molecules of Hela cells with and without irradiation were quantified as
403,70026,400 and 212,70012,000 per cell, respectively [30]
Flow cytometry CD5, CD10, CD11c, CD19, CD20, CD22, CD23, and CD79b molecules of
positive single B cells were quantified as 8.7 (normal), 13.4 (CLL), and 6.0
(B-ALL); 10.6 (normal), 11.4 (CLL), 21.7 (B-ALL), and 23.9 (HCL); 10.4
(normal), 5.4 (CLL), 2.3 (B-All), and 49.3 (HCL); 21.5 (normal), 13.3 (CLL),
12.5 (B-All), and 25.5 (HCL); 53.9 (normal), 10.5 (CLL), 14.3 (B-All), and
66.6 (HCL); 14.3 (normal), 2.4 (CLL), 4.0 (B-All), and 12.7 (HCL); 7.2
(normal), 4.5 (CLL), 0.2 (B-All), and 5.9 (HCL); and 13.9 (normal), 8.8 (CLL),
9.6 (B-All), and 14.8 (HCL) ( 103 )[31]
Flow cytometry Glycophorin A molecules of red blood cells were quantified as in the range of
3–13 105 per cell [32]
Flow cytometry FY1, FY2, MNS3, and MNS4 molecules of single red blood cells were quantified as
11,332 (524–26,918), 7204 (4405–11,988), 10,387 (149–68,819), and
100,589 (2638–282,788) per cell, respectively [33]
Flow cytometry CD20 molecules of chronic lymphocytic leukemia were quantified as 9341
(1039–123,045) for 17p depletion, 5886 (2248–26,221) for 11q depletion,
23,603 (1688–112,301) for trisomy, 8828 (2420–122,875) for negative FISH,
and 10,781 (1711–98,361) for 13q depletion (per cell) [34]
Flow cytometry Met1, Met2, Her2, and Her3 of cell lines including T47D, H441, Du145, HT29,
and A549 were quantified as NA, ND, 80,67714,938, and 50801641;
109,90936,334, 137,25242,899, 66,91311,847, and 82971932;
7000 1057, 11,2112009, 20,5242317, 2751429; 94402219,
15,4601898, 45,0291683, and 402086; and 37,2246878,
45,0966530, 12,2193157, and 1498266 (per cell) [35]
Flow cytometry CD5 molecules of B cells of normal people, CLL, atypical CLL, MCL, MZL, HCL,
LPL, and FL were quantified as 315142 (145–586), 8877 5708
(169–21,017), 44754141 (396–11,536), 53713331 (126–10,254),
234 253 (19–770), 178139 (96–338), 140139 (34–344), and 46 17
(29–82) (per cell) [36]
Flow cytometry Intracellular Bcl-2 molecules of tumor cell lines of HL-60, K562, DOHH2, Jurkat,
MCF7, MDA435/LCC6, and PBL were quantified as 12.66.5, 32.918,
200 58.3, 65.14.6, 97.423.1, 52.97.3, and 38.51.7 (10^3 per cell)
[37]
Flow cytometry Intracellular perforin molecules of natural killer cells of healthy controls and
individuals with heterozygous and homozygous perforin deficiencies were
quantified as 35611175, 2260, and 212, respectively (per cell) [38]
Flow cytometry Intracellular proteins of ZAP-70 were quantified as 12,621 (normal B cells) and
102,615119,252 and 233,259182,071 from CD19+/CD5+ and CD3+/
CD45+ B cells from chronic lymphocytic leukemia (per cell) [39]
Mass cytometry CD117, CD34, CD133, and CD45 of progenitors of normal bone marrows were
quantified as 2.8 104 , 6.1 104 , and 4.1 104 , 2.0 105 per cell, while
CD95, CD45, CD38, CD15, and CD11 of granulocytes/monocytes of normal
bone marrows were quantified as 3.1 105 , 4.1 104 , 7.0 104 , 1.8 105 , and
1.6 105 per cell [40]
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298 Beiyuan Fan et al.

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