13-parameter flow cytometry: identification
of human naive T cells by phenotype, func-
tion, and T-cell receptor diversity. Nat Med 7
(2):245–248. https://doi.org/10.1038/
84701
- Perfetto SP, Chattopadhyay PK, Roederer M
(2004) Seventeen-colour flow cytometry:
unravelling the immune system. Nat Rev
Immunol 4(8):648–655. https://doi.org/
10.1038/nri1416
- Herzenberg LA, Parks D, Sahaf B, Perez O,
Roederer M, Herzenberg LA (2002) The his-
tory and future of the fluorescence activated
cell sorter and flow cytometry: a view from
stanford. Clin Chem 48(10):1819–1827
- Zenger VE, Vogt R, Mandy F, Schwartz A,
Marti GE (1998) Quantitative flow cytome-
try: inter-laboratory variation. Cytometry 33
(2):138–145. https://doi.org/10.1002/(
SICI)1097-0320(19981001)33:2<138::
AID-CYTO8>3.0.CO;2-F
- Schwartz A, Fernandez-Repollet E (2001)
Quantitative flow cytometry. Clin Lab Med
21(4):743–761
- Marti GE, Zenger VE, Vogt R, Gaigalas A
(2002) Quantitative flow cytometry: history,
practice, theory, consensus, inter-laboratory
variation and present status. Cytotherapy 4
(1):97–98. https://doi.org/10.1080/
146532402317251626
- Maher KJ, Fletcher MA (2005) Quantitative
flow cytometry in the clinical laboratory. Clin
Appl Immunol Rev 5(6):353–372.https://
doi.org/10.1016/j.cair.2005.10.001
- Atkuri KR, Stevens JC, Neubert H (2015)
Mass cytometry: a highly multiplexed single-
cell technology for advancing drug develop-
ment. Drug Metab Dispos 43(2):227–233.
https://doi.org/10.1124/dmd.114.060798
- Spitzer Matthew H, Nolan Garry P (2016)
Mass cytometry: single cells, many features.
Cell 165(4):780–791. https://doi.org/10.
1016/j.cell.2016.04.019
- Comi TJ, Do TD (2017) Categorizing cells
on the basis of their chemical profiles: prog-
ress in single-cell mass spectrometry. J Am
Chem Soc 139(11):3920–3929.https://doi.
org/10.1021/jacs.6b12822
- Bendall SC, Nolan GP, Roederer M, Chatto-
padhyay PK (2012) A deep profiler’s guide to
cytometry. Trends Immunol 33(7):323–332.
https://doi.org/10.1016/j.it.2012.02.010
- Bjornson ZB, Nolan GP, Fantl WJ (2013)
Single-cell mass cytometry for analysis of
immune system functional states. Curr Opin
Immunol 25(4):484–494.https://doi.org/
10.1016/j.coi.2013.07.004
56. Ornatsky OI, Kinach R, Bandura DR, Lou X,
Tanner SD, Baranov VI, Nitz M, Winnik MA
(2008) Development of analytical methods
for multiplex bio-assay with inductively cou-
pled plasma mass spectrometry. J Anal At
Spectrom 23(4):463–469. https://doi.org/
10.1039/b710510j
57. Ornatsky OI, Lou X, Nitz M, Schafer S, Shel-
drick WS, Baranov VI, Bandura DR, Tanner
SD (2008) Study of cell antigens and intracel-
lular DNA by identification of element-
containing labels and metallointercalators
using inductively coupled plasma mass spec-
trometry. Anal Chem 80(7):2539–2547.
https://doi.org/10.1021/ac702128m
58. Bandura DR, Baranov VI, Ornatsky OI,
Antonov A, Kinach R, Lou X, Pavlov S,
Vorobiev S, Dick JE, Tanner SD (2009)
Mass cytometry: technique for real time single
cell multitarget immunoassay based on induc-
tively coupled plasma time-of-flight mass
spectrometry. Anal Chem 81
(16):6813–6822.https://doi.org/10.1021/
ac901049w
59. Bendall SC, Simonds EF, Qiu P, Amir el AD,
Krutzik PO, Finck R, Bruggner RV,
Melamed R, Trejo A, Ornatsky OI, Balderas
RS, Plevritis SK, Sachs K, Pe’er D, Tanner SD,
Nolan GP (2011) Single-cell mass cytometry
of differential immune and drug responses
across a human hematopoietic continuum.
Science 332(6030):687–696. https://doi.
org/10.1126/science.1198704
60. Newell Evan W, Sigal N, Bendall Sean C,
Nolan Garry P, Davis Mark M (2012) Cyto-
metry by time-of-flight shows combinatorial
cytokine expression and virus-specific cell
niches within a continuum of CD8+ T cell
phenotypes. Immunity 36(1):142–152.
https://doi.org/10.1016/j.immuni.2012.
01.002
61. Horowitz A, Strauss-Albee DM, Leipold M,
Kubo J, Nemat-Gorgani N, Dogan OC, Dek-
ker CL, Mackey S, Maecker H, Swan GE,
Davis MM, Norman PJ, Guethlein LA,
Desai M, Parham P, Blish CA (2013) Genetic
and environmental determinants of human
NK cell diversity revealed by mass cytometry.
Sci Transl Med 5(208):208ra145. https://
doi.org/10.1126/scitranslmed.3006702
62. Newell EW, Sigal N, Nair N, Kidd BA, Green-
berg HB, Davis MM (2013) Combinatorial
tetramer staining and mass cytometry analysis
facilitate T-cell epitope mapping and charac-
terization. Nat Biotechnol 31(7):623–629.
https://doi.org/10.1038/nbt.2593
63. Tanner SD, Baranov VI, Ornatsky OI, Ban-
dura DR, George TC (2013) An introduction
Single-Cell Protein Assays: A Review 305
