integrated single-cell pipettes. J Am Chem
Soc 136(31):10858–10861. https://doi.
org/10.1021/ja5053279
- Frohlich J, Konig H (2000) New techniques
for isolation of single prokaryotic cells. FEMS
Microbiol Rev 24(5):567–572
- Voet T, Kumar P, Van Loo P, Cooke SL,
Marshall J, Lin ML, Esteki MZ, Van der Aa
N, Mateiu L, McBride DJ, Bignell GR, McLa-
ren S, Teague J, Butler A, Raine K, Stebbings
LA, Quail MA, D’Hooghe T, Moreau Y,
Futreal PA, Stratton MR, Vermeesch JR,
Campbell PJ (2013) Single-cell paired-end
genome sequencing reveals structural varia-
tion per cell cycle. Nucleic Acids Res 41
(12):6119–6138.https://doi.org/10.1093/
nar/gkt345
- Klitgaard K, Jensen TK, Angen O, Boye M
(2007) Measurement of bacterial gene expres-
sion in vivo by laser capture microdissection
and quantitative real-time RT-PCR. J Micro-
biol Methods 69(2):414–416.https://doi.
org/10.1016/j.mimet.2006.12.003
- Fink L, Kwapiszewska G, Wilhelm J, Bohle
RM (2006) Laser-microdissection for cell
type- and compartment-specific analyses on
genomic and proteomic level. Exp Toxicol
Pathol 57:25–29.https://doi.org/10.1016/
j.etp.2006.02.010
- Vannucci FA, Foster DN, Gebhart CJ (2013)
Laser microdissection coupled with RNA-seq
analysis of porcine enterocytes infected with
an obligate intracellular pathogen (Lawsonia
intracellularis). BMC Genomics 14.https://
doi.org/10.1186/1471-2164-14-421
- Nakamura N, Ruebel K, Jin L, Qian X, Zhang
H, Lloyd RV (2007) Laser capture microdis-
section for analysis of single cells. Methods
Mol Med 132:11–18
- Huang G, Wang S (2013) Establishment of a
new method to detect gene expression by
laser capture microdissection-assisted single-
cell real time RT-PCR without RNA purifica-
tion. Mol Biol 47(4):509–514.https://doi.
org/10.1134/S0026893313040055
- Vandewoestyne M, Deforce D (2010) Laser
capture microdissection in forensic research: a
review. Int J Legal Med 124(6):513–521.
https://doi.org/10.1007/s00414-010-
0499-4
- Decarlo K, Emley A, Dadzie OE, Mahalingam
M (2011) Laser capture microdissection:
methods and applications. Methods Mol Biol
755:1–15.https://doi.org/10.1007/978-1-
61779-163-5_1
- Fuller SA, Takahashi M, Hurrell JG (2001)
Cloning of hybridoma cell lines by limiting
dilution. Curr Protoc Mol Biol Chapter 11:
Unit 11 18. https://doi.org/10.1002/
0471142727.mb1108s01
- Wang Z, Gerstein M, Snyder M (2009) RNA-
Seq: a revolutionary tool for transcriptomics.
Nat Rev Genet 10(1):57–63. https://doi.
org/10.1038/nrg2484
- Tang FC, Barbacioru C, Wang YZ, Nordman
E, Lee C, Xu NL, Wang XH, Bodeau J, Tuch
BB, Siddiqui A, Lao KQ, Surani MA (2009)
mRNA-Seq whole-transcriptome analysis of a
single cell. Nat Methods 6(5):377–U386.
https://doi.org/10.1038/Nmeth.1315
- Trapnell C, Cacchiarelli D, Grimsby J,
Pokharel P, Li SQ, Morse M, Lennon NJ,
Livak KJ, Mikkelsen TS, Rinn JL (2014) The
dynamics and regulators of cell fate decisions
are revealed by pseudotemporal ordering of
single cells. Nat Biotechnol 32
(4):381–U251. https://doi.org/10.1038/
nbt.2859
- Wills QF, Livak KJ, Tipping AJ, Enver T,
Goldson AJ, Sexton DW, Holmes C (2013)
Single-cell gene expression analysis reveals
genetic associations masked in whole-tissue
experiments. Nat Biotechnol 31(8):748.
https://doi.org/10.1038/nbt.2642
- Islam S, Kjallquist U, Moliner A, Zajac P, Fan
JB, Lonnerberg P, Linnarsson S (2011) Char-
acterization of the single-cell transcriptional
landscape by highly multiplex RNA-seq.
Genome Res 21(7):1160–1167.https://doi.
org/10.1101/gr.110882.110
- Islam S, Kjallquist U, Moliner A, Zajac P, Fan
JB, Lonnerberg P, Linnarsson S (2012)
Highly multiplexed and strand-specific sin-
gle-cell RNA 5^0 end sequencing. Nat Protoc
7(5):813–828. https://doi.org/10.1038/
nprot.2012.022
- Islam S, Zeisel A, Joost S, La Manno G, Zajac
P, Kasper M, Lonnerberg P, Linnarsson S
(2014) Quantitative single-cell RNA-seq
with unique molecular identifiers. Nat Meth-
ods 11(2):163–166. https://doi.org/10.
1038/nmeth.2772
- Hashimshony T, Wagner F, Sher N, Yanai I
(2012) CEL-seq: single-cell RNA-seq by mul-
tiplexed linear amplification. Cell Rep 2
(3):666–673.https://doi.org/10.1016/j.cel
rep.2012.08.003
- Ramskold D, Luo SJ, Wang YC, Li R, Deng
QL, Faridani OR, Daniels GA, Khrebtukova
I, Loring JF, Laurent LC, Schroth GP, Sand-
berg R (2012) Full-length mRNA-Seq from
single-cell levels of RNA and individual circu-
lating tumor cells. Nat Biotechnol 30
(8):777–782.https://doi.org/10.1038/nbt.
2282
Applications of Single-Cell Sequencing for Multiomics 365
