Computational Systems Biology Methods and Protocols.7z

Table 2Results from Ion Torrent PGM of seven samples mixed togetherLibraryname

a

Total numberof reads

Unique mappedreads (%)

Error (%) Sequence depth

Coveragepercentage (

>

2)

b

Coveragepercentage (

>

10)

c

Chimericreads (%)

R.sp.1_P_480

30,453

90.93

0.54

426.8

>

95

d

>

95

d

0.74

R.sp.2_P_480

33,259

97.22

0.53

502.5

100

100

2.91

O.sp._P_480

37,450

98.34

0.59

685.4

100

100

1.33

R.sp.2_D_480 21,369

11.49

0.57

36.5

86.97

78.62

0.11

R.sp.2_D_2kb 45,605

21.32

0.63

80.9

99.49

95.42

0.73

O.sp._D_480

24,727

15.65

0.49

60.8

83.12

66.5

0.1

O.sp._D_2kb

84,599

18.25

0.62

231.4

100

100

0.12

aThere are three parameters combined with underline in the library name: species name, source DNA, and library length. R.sp.1 represents

Rana

sp.1; R.sp.2 represents

Rana

sp.2; O.sp. represents

Onychodactylus

sp

.; P represents using PCR products to construct library; D represents using total DNA. The number 480 represents the length of 480 base

pairs of the libraries; 2 kb represents the length of 2000 base pairs of the librariesbSites with

>

2 of depth sequence are included to statistic genome coverage

cSites with depth sequence

>

10 are included to statistic genome coverage

dThe mitochondrial genome of

Rana

sp.1 was incompletely recovered for its control region which had long repetitive DNA

Capture Hybridization of Long-Range DNA Fragments for High-Throughput Sequencing 39