Systematic large-scale meta-analysis identifies a
panel of two mRNAs as blood biomarkers for
colorectal cancer detection. Oncotarget 7
(21):30295–30306. https://doi.org/10.
18632/oncotarget.8108- Allen-Mersh TG, McCullough TK, Patel H,
Wharton RQ, Glover C, Jonas SK (2007)
Role of circulating tumour cells in predicting
recurrence after excision of primary colorectal
carcinoma. Br J Surg 94(1):96–105.https://
doi.org/10.1002/bjs.5526 - Katsuno H, Zacharakis E, Aziz O, Rao C,
Deeba S, Paraskeva P, Ziprin P, Athanasiou T,
Darzi A (2008) Does the presence of circulat-
ing tumour cells in the venous drainage of
curative colorectal cancer resections determine
prognosis? A meta-analysis. Ann Surg Oncol
15(11):3083–3091. https://doi.org/10.
1245/s10434-008-0131-8 - El-Hefnawy T, Raja S, Kelly L, Bigbee WL,
Kirkwood JM, Luketich JD, Godfrey TE
(2004) Characterization of amplifiable, circu-
lating RNA in plasma and its potential as a tool
for cancer diagnostics. Clin Chem 50
(3):564–573. https://doi.org/10.1373/
clinchem.2003.028506 - Bao Y, Ching B, Mouanoutoua M, Yu W,
Keys D, Desai S, Stevens J (2012) Cancer bio-
marker research using castPCR technology.
AACR - Wilkening S, Hemminki K, Thirumaran RK,
Bermejo JL, Bonn S, Fo ̈rsti A, Kumar R
(2005) Determination of allele frequency in
pooled DNA: comparison of three PCR-based
methods. BioTechniques 39(6):853–858 - Goto K, Ichinose Y, Ohe Y, Yamamoto N,
Negoro S, Nishio K, Itoh Y, Jiang H,
Duffield E, McCormack R, Saijo N, Mok T,
Fukuoka M (2012) Epidermal growth factor
receptor mutation status in circulating free
DNA in serum: from IPASS, a phase III study
of gefitinib or carboplatin/paclitaxel in
non-small cell lung cancer. J Thorac Oncol 7
(1):115–121.https://doi.org/10.1097/JTO.
0b013e3182307f98 - Karachaliou N, Mayo-de las Casas C,
Queralt C, de Aguirre I, Melloni B,
Cardenal F, Garcia-Gomez R, Massuti B, Sa ́n-
chez JM, Porta R, Ponce-Aix S, Moran T,
Carcereny E, Felip E, Bover I, Insa A,
Reguart N, Isla D, Vergnenegre A, de
Marinis F, Gervais R, Corre R, Paz-Ares L,
Morales-Espinosa D, Viteri S,
Drozdowskyj A, Jordana-Ariza N, Ramirez-
Serrano JL, Molina-Vila MA, Rosell R, Spanish
Lung Cancer Group (2015) Association of
EGFR L858R mutation in circulating free
DNA with survival in the EURTAC trial.
JAMA Oncol 1(2):149–157.https://doi.org/
10.1001/jamaoncol.2014.257- Hindson BJ, Ness KD, Masquelier DA,
Belgrader P, Heredia NJ, Makarewicz AJ,
Bright IJ, Lucero MY, Hiddessen AL, Legler
TC, Kitano TK, Hodel MR, Petersen JF, Wyatt
PW, Steenblock ER, Shah PH, Bousse LJ,
Troup CB, Mellen JC, Wittmann DK, Erndt
NG, Cauley TH, Koehler RT, So AP, Dube S,
Rose KA, Montesclaros L, Wang S, Stumbo
DP, Hodges SP, Romine S, Milanovich FP,
White HE, Regan JF, Karlin-Neumann GA,
Hindson CM, Saxonov S, Colston BW (2011)
High-throughput droplet digital PCR system
for absolute quantitation of DNA copy num-
ber. Anal Chem 83(22):8604–8610.https://
doi.org/10.1021/ac202028g - Taniguchi K, Uchida J, Nishino K, Kumagai T,
Okuyama T, Okami J, Kato K (2011) Quanti-
tative detection of EGFR mutations in circulat-
ing tumour DNA derived from lung
adenocarcinomas. Clin Cancer Res 17
(24):7808–7815. https://doi.org/10.1158/
1078-0432.CCR-11-1712 - Taniguchi K, Uchida J, Nishino K, Kumagai T,
Okuyama T, Okami J, Higashiyama M,
Kodama K, Imamura F, Kato K (2012) Nonin-
vasive identification and monitoring of cancer
mutations by targeted deep sequencing of
plasma DNA. Sci Transl Med 4
(136):136ra168-136ra168. https://doi.org/
10.1158/1078-0432.CCR-11-1712 - Kinde I, Wu J, Papadopoulos N, Kinzler KW,
Vogelstein B (2011) Detection and quantifica-
tion of rare mutations with massively parallel
sequencing. Proc Natl Acad Sci 108
(23):9530–9535. https://doi.org/10.1073/
pnas.1105422108 - Newman AM, Bratman SV, To J, Wynne JF,
Eclov NC, Modlin LA, Liu CL, Neal JW,
Wakelee HA, Merritt RE, Shrager JB, Loo
BW Jr, Alizadeh AA, Diehn M (2014) An ultra-
sensitive method for quantitating circulating
tumour DNA with broad patient coverage.
Nat Med 20(5):548–554. https://doi.org/
10.1038/nm.3519 - Griffin TJ, Smith LM (2000) Single-nucleotide
polymorphism analysis by MALDI–TOF mass
spectrometry. Trends Biotechnol 18(2):77–84 - Giannini R, Lupi C, Sensi E, Alı`G, Proietti A,
Boldrini L, Servadio A, Giordano M,
Macerola E, Bruno R, Borrelli N, Chella A,
Melfi F, Lucchi M, Ribechini A, Vasile E,
Cappuzzo F, Mussi A, Fontanini G (2016)
EGFR and KRAS mutational analysis in a
large series of Italian non-small cell lung cancer
patients: 2,387 cases from a single center.
62 Jun Li et al.