Produce Degradation Pathways and Prevention

(Romina) #1

8 Produce Degradation: Reaction Pathways and their Prevention


TABLE 1.1Commonly Used PCR Primer Sets for Identification and Detection of Soft Rot ErwiniaPrimer Name

Primer Sequence

Closest BLASTn Hit

a

Specificity

Amplicon Size (bp)

Source

Y1

5 ′
ttaccggacg ccgagctgtg gcgt 3


Erwinia carotovora

pel

B gene ...

Erwinia carotovora

434

Darrasse et al., 1994

Y2

5 ′
caggaagatg tcgttatcgc gagt 3


Erwinia carotovora

pel

B gene ...

y45

5 ′
tcaccggacg ccgaactgtg gcgt 3


Erwinia carotovora

pel

B gene ...

Erwinia carotovora
subsp.

atroseptica

439

Frechon et al., 1995

y46

5 ′
tcgccaacgt tcagcagaac aagt 3


Erwinia carotovora

pel

B gene ...

ECA1f

5 ′
cggcatcata aaaacacg 3


(Pan troglodytes

BAC clone RP43-4 ...)

Erwinia carotovora
subsp.

atroseptica

690

De Boer and Ward, 1995

ECA1r

5 ′
gcacacttca tccagcga 3


(Homo sapiens

chromosome 8, clone ... )

ADE1

5 ′
gatcagaaag cccgcagcca gat 3


Erwinia chrysanthemi

pel

E

Erwinia chrysanthemi

420

Nassar et al., 1996

ADE2

5 ′
ctgtggccga tcaggatggt tttgtcgtgc 3


Erwinia chrysanthemi

pel

E

ERWFOR

5 ′
acgcatgaaa tcttccaatg c 3


(Homo sapiens

BAC clone RP11-391J13)

Erwinia carotovora
subsp.

atroseptica

389

Smid et al., 1995

ATRREV

5 ′
atcgatattt gattgtc 3


(Arabidopsis thaliana

DNA)

ERWFOR

5 ′
acgcatgaaa tcttccaatg c 3


(Homo sapiens

BAC clone RP11-391J13)

Erwinia chrysanthemi

450

Smid et al., 1995

CHRREV

5 ′
agtgctgccg tacagcacgt 3


Erwinia chrysanthemi

prt

a
Results from BALSTn search using GenBank nucleotide sequence database (http://www.ncbi.nlm.nih.gov). Description in parenthesis

may not infer true genetic

relationship.
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