8 Produce Degradation: Reaction Pathways and their Prevention
TABLE 1.1Commonly Used PCR Primer Sets for Identification and Detection of Soft Rot ErwiniaPrimer NamePrimer SequenceClosest BLASTn HitaSpecificityAmplicon Size (bp)SourceY15 ′
ttaccggacg ccgagctgtg gcgt 3′Erwinia carotovorapelB gene ...Erwinia carotovora434Darrasse et al., 1994Y25 ′
caggaagatg tcgttatcgc gagt 3′Erwinia carotovorapelB gene ...y455 ′
tcaccggacg ccgaactgtg gcgt 3′Erwinia carotovorapelB gene ...Erwinia carotovora
subsp.atroseptica439Frechon et al., 1995y465 ′
tcgccaacgt tcagcagaac aagt 3′Erwinia carotovorapelB gene ...ECA1f5 ′
cggcatcata aaaacacg 3′(Pan troglodytesBAC clone RP43-4 ...)Erwinia carotovora
subsp.atroseptica690De Boer and Ward, 1995ECA1r5 ′
gcacacttca tccagcga 3′(Homo sapienschromosome 8, clone ... )ADE15 ′
gatcagaaag cccgcagcca gat 3′Erwinia chrysanthemipelEErwinia chrysanthemi420Nassar et al., 1996ADE25 ′
ctgtggccga tcaggatggt tttgtcgtgc 3′Erwinia chrysanthemipelEERWFOR5 ′
acgcatgaaa tcttccaatg c 3′(Homo sapiensBAC clone RP11-391J13)Erwinia carotovora
subsp.atroseptica389Smid et al., 1995ATRREV5 ′
atcgatattt gattgtc 3′(Arabidopsis thalianaDNA)ERWFOR5 ′
acgcatgaaa tcttccaatg c 3′(Homo sapiensBAC clone RP11-391J13)Erwinia chrysanthemi450Smid et al., 1995CHRREV5 ′
agtgctgccg tacagcacgt 3′Erwinia chrysanthemiprta
Results from BALSTn search using GenBank nucleotide sequence database (http://www.ncbi.nlm.nih.gov). Description in parenthesismay not infer true geneticrelationship.