54 A. Gabibov et al.
〈〉Fig. 4.5.Kinetic analysis of the scDNA transformations catalysed by topoisomerase
I. (a) Computer simulations of the distribution of topoisomers of pTM plasmid
induced by topoisomerase I. The following parameters were taken.kcl=0.06s−^1 ,
kl=0.6s−^1 ,kr=1s−^1 ,kd=10−^6. DNA Hooks constantB=1, 100 RT.(b)〈σ〉vs
time dependence calculated according to formula (4.16). (c)ρvs time dependence
calculated according to formula (4.17)
4.2.3 Interaction of scDNA with Eukaryotic DNA Topoisomerases
It is expedient to consider what kinds of LD signal shifts were observed in
the process of pTM and BlueScript plasmid relaxation catalysed by human
topoisomerase I. Some curves typical for the experiments are seen in Fig. 4.7.
It is evident that the interaction between topoisomerase I and either of the
plasmids results in two different pictures. In experiments with a pTM plasmid
the linear dichroism of the reaction mixture rose fast. The evidence was also
furnished by electrophoresis assay proofs showing that the signal shift was