Science - USA (2022-06-03)

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SUPPLEMENTARY MATERIALS

science.org/doi/10.1126/science.abm1072

Materials and Methods

Figs. S1 to S15

Tables S1 to S17

References ( 27 – 44 )

MDAR Reproducibility Checklist

Submitted 26 August 2021; accepted 6 April 2022

10.1126/science.abm1072

Kearneyet al., Science 376 , 1110–1114 (2022) 3 June 2022 4of4

42 44 46 48 50 52

°C

critical thermal maximum (w < v < f)

42 44 46 48 50 52

n = 36

n = 23

n = 53

whitei

virgo

flavolineata

0 5 10 15

minutes

chill coma recovery (w = v = f)

0 5 10 15

n = 46

n = 79

n = 90

0 102030405060

gH 2 O mg / h

water loss rate (w > v < f)

0 102030405060

n = 30

n = 20

n = 49

0.0 0.5 1.0 1.5 2.0

log(lCO 2 mg / h)

metabolic rate (w = v = f)

0.0 0.5 1.0 1.5 2.0

n = 30

n = 20

n = 49

2000 4000 6000 80002000 4000 6000 8000 1200012000

K

Arrhenius temperature (w = v = f)

n = 29

n = 19

n = 42

50 100 150 200 250 300

mg

female mass (w < v = f)

50 100 150 200 250 300

n = 73

n = 80

n = 82

12345

mg

egg mass (w < v > f)

12345

n = 76

n = 158

n = 101

2468

mg

hatchling mass (w < v > f)

2468

n = 143

n = 526

n = 316

0 5 10 15 20 25 30

# eggs

clutch size (w > v < f)

0 5 10 15 20 25 30

n = 219

n = 727

n = 466

01020304050

mg

clutch mass (w < v > f)

01020304050

n = 18

n = 25

n = 41

0 50 100 150

# eggs

lifetime reproduction (w > v = f)

0 50 100 150

n = 84

n = 342

n = 222

30 40 50 60 70

days

development time (w = v < f)

30 40 50 60 70

n = 12

n = 39

n = 92

40 60 80 100

days

time to maturity (w = v = f)

40 60 80 100

n = 28

n = 227

n = 64

0 50 100 150 200 250 300

days

longevity (w = v = f)

0 50 100 150 200 250 300

n = 28

n = 227

n = 64

A C E G I K M

B D F H J L N

Fig. 4. Laboratory phenotypic comparisons (trait medians, quartiles, and extremes) of physiological
and life history traits between the parthenogeneticW. virgoand its sexual progenitorsW. whiteiand
W. flavolineata. Sample size is indicated byn, and directions of significant differences [investigated by
analysis of variance (ANOVA) or repeated-measures ANOVA; see the materials and methods] are indicated in
the heading of each subplot: w,W. whitei,f, W. flavolineata, and v,W. virgo. Light gray dots are individual
data points. Red dots in the clutch size plot are for the southernmost populations of the sexual species,
where they geographically meet the parthenogens. Arrhenius temperature refers to the temperature
sensitivity of the metabolic rate.

RESEARCH | REPORT