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ACKNOWLEDGMENTS

We thank the staff of the Cryo-EM Core and the Computing Core of

Westlake University for technical support.Funding:This work was

supported by the National Natural Science Foundation of China

(grant 31930059 to Y.S.), Westlake University (start-up funds

to Y.S.), The China Postdoctoral Science Foundation (grant

2021M692888 to X. Zhan), and the National Postdoctoral Program

for Innovative Talents of China (grant BX2021268 to X. Zhan).

Author contributions:Y.S. conceived and supervised the project.

X. Zhu, P.W., and C.Z. prepared the sample. G.H., C.Z., X. Zhan,

K.L., and J.L. collected the EM data. G.H., C.Z., X. Zhan, and

K.L. processed the EM data. G.H. performed the cryo-EM SPA

calculation. X. Zhan built the models. Q.Z., C.Y., Q.T., and M.L.

provided critical support. All authors analyzed the structure.

G.H., C.Z., X. Zhan, and Y.S. wrote the manuscript.Competing

interests:The authors declare no competing interests.Data and

materials availability:The atomic coordinates of the CR subunit

and NTD2 of Nup358 have been deposited in the PDB with

accession codes 7FIK and 7FIL, respectively. The EM maps for the

CR core region, the Nup358 region, and the NTD2 for Nup358 have

been deposited in the Electron Microscopy Data Bank with the

accession codes EMD-31600, EMD-31601, and EMD-31602,

respectively.License information:Copyright © 2022 the authors,

some rights reserved; exclusive licensee American Association

for the Advancement of Science. No claim to original US

government works.https://www.science.org/about/science-

licenses-journal-article-reuse

SUPPLEMENTARY MATERIALS

science.org/doi/10.1126/science.abl8280

Figs. S1 to S18

Tables S1 to S5

MDAR Reproducibility Checklist

View/request a protocol for this paper fromBio-protocol.

Submitted 7 August 2021; resubmitted 30 September 2021

Accepted 10 May 2022

10.1126/science.abl8280

Zhuet al., Science 376 , eabl8280 (2022) 10 June 2022 10 of 10

RESEARCH | STRUCTURE OF THE NUCLEAR PORE