Fontanaet al., Science 376 , eabm9326 (2022) 10 June 2022 3of11
Fig. 2. Fitting of Y-complex Nups with AlphaFold.(A) Cryo-EM density
(contour level, 8.0s) of the outer Y-complex colored by individual Nups.
Theb-propeller domain of Nup133 was not built because of lack of density.
(B) Two views of superimposed inner Y-complex (blue) and outer Y-complex
(green) by the two short arms of the Y-complexes. The distal ends of
aligned Nup133 without counting theb-propeller have a distance of ~38 Å.
(C) Comparison of AlphaFold prediction (left) and homology modeling (right)
for Nup160. The cryo-EM density (contour level, 4.5s) and the positioning
of Nup160 (yellow) and Nup96 (cyan) by the two predictions are shown
at bottom. (D andE) AlphaFold-generated model of the Nup85-Seh1 complex
fitted with (D) the cryo-EM density (contour level, 4.5s) and shown to
highlight the inserted blade.RESEARCH | STRUCTURE OF THE NUCLEAR PORE