Fontanaet al., Science 376 , eabm9326 (2022) 10 June 2022 5of11
Fig. 3. Interactions mediated by Nup205 and the Nup214-Nup88-Nup62
complex.(A) Overall interactions of inner Nup205 (orange) and outer Nup205
(yellow) with Y-complexes. Outer Nup205 directly interacts with Nup160,
Nup85, and Seh1 of the outer Y-complex and with Nup43 of the inner
Y-complex. The inner Nup205 directly interacts with Nup160 of the inner
Y-complex, C-terminal region of Nup155, and Nup88b-propeller in the
Nup214-Nup88-Nup62 complexes. The dashed arrows indicate the locational
difference between inner and outer Nup205. (B) Superposition of the inner
(blue) and outer (green) Y-complexes together with the bound Nup205
molecules, showing the positions of the inner (orange) and outer (yellow)
Nup205 relative to Nup85 and Nup160. The N-terminal region of Nup205
binds similarly to inner and outer Nup160 molecules; the C-terminal
domain binds outer Nup85 but pivots away from the inner Nup85 because
of the presence of the Nup214-Nup88-Nup62 complexes. (C) Overview
(left) and fitting (right) of the AlphaFold-predicted one Nup214-Nup88-Nup62
complex into the cryo-EM density map of NPC CR monomer (contour
level, 4.5s). ( D) Overview (left) and fitting (right) of the AlphaFold-predicted
Nup214-Nup88-Nup62 complexes into the cryo-EM density map (contour
level, 4.5s), with the neighboring inner Nup85. Two Nup214-Nup88-Nup62
complexes are shown.RESEARCH | STRUCTURE OF THE NUCLEAR PORE