conceptualized the study, supervised the project, and wrote the
manuscript. CRediT roles: Conceptualization: S.M., A.O.-K., G.H.,
J.K., and M.B. Methodology: S.M., A.O.-K., B.T., M.S., and J.K.
Investigation: S.M., A.O.-K., M.S., R.T., B.T., C.E.Z., K.B., F.H.S.,
E.M., M.-T.M., W.H., G.H., J.K., and M.B. Visualization: S.M., A.O.-K.,
M.S., R.T., C.E.Z., M.-T.M., and J.K. Funding acquisition: M.B.
Project administration: G.H., J.K., and M.B. Supervision: S.M.,
G.H., J.K., and M.B. Writing–original draft: S.M., M.S., G.H.,
M.B., and J.K. Writing–review & editing: S.M., A.O.-K., M.S., G.H.,
M.B., and J.K.Competing interests:The authors declare that
they have no competing interests.Data and materials
availability:EM maps associated with the manuscript have been
deposited in the Electron Microscopy Data Bank (EMDB) under
accession codes EMD-14243, -14321, -14322, -14325, -14326,
-14327, -14328, and -14330. The models have been deposited into
the Protein Data Bank (PDB) under accession codes 7R1Y, 7R5K,
and 7R5J. The predicted local quality scores (pLDDT) have been
reported in the B-factor property of the deposited models.
Software code for subtomogram averaging and geometric cleaning
is available at Zenodo ( 71 ). License information:Copyright ©
2022 the authors, some rights reserved; exclusive licensee
American Association for the Advancement of Science. No claim to
original US government works.https://www.science.org/about/
science-licenses-journal-article-reuseSUPPLEMENTARY MATERIALS
science.org/doi/10.1126/science.abm9506
Supplementary Text
Figs. S1 to S29
Tables S1 to S6
References ( 106 – 129 )
MDAR Reproducibility Checklist
View/request a protocol for this paper fromBio-protocol.Submitted 24 October 2021; accepted 22 April 2022
10.1126/science.abm9506Mosalagantiet al., Science 376 , eabm9506 (2022) 10 June 2022 13 of 13
RESEARCH | STRUCTURE OF THE NUCLEAR PORE