reSeArcH Article
Extended Data Fig. 3 | Local resolution maps of the eight
reconstructions. a, c, e, g, i, k, l, n, The local resolution maps estimated
with RELION 2.0. All electron microscopy maps were generated in
Chimera and contoured at levels of 0.027 (a), 0.022 (c), 0.023 (e), 0.021
(g), 0.02 (i), 0.021 (k), 0.015 (l) and 0.021 (n). b, Electron microscopy map
of apo-CaM from the reconstruction shown in a. d, Electron microscopy
map of CaM-M. f, h, The electron microscopy densities of Ca^2 +-CaM
were generated from the maps of FKBP12.6/ATP/caffeine/low-[Ca^2 +]/
Ca^2 +-CaM and CHAPS- and DOPC-treated FKBP12.6/ATP/caffeine/
low-[Ca^2 +]/Ca^2 +-CaM that were low-pass-filtered to 5.6 Å with contour
levels of 0.015 (f) and 0.013 (h), respectively. j, m, Electron microscopy
densities of Ca^2 +-CaM in FKBP12.6/ATP/caffeine/high-[Ca^2 +]/Ca^2 +-CaM
(j) and PCB95/low-[Ca^2 +]/Ca^2 +-CaM (m). The densities of both lobes
were resolved in the map, although the N-lobe was resolved better than the
C-lobe.