Nature - 15.08.2019

Letter reSeArCH

HEK293T cells-GFP IP from 10 cm dish

GFP_1GFP_2GFP_3 GFP-SidJ_1GFP-SidJ_2GFP-SidJ_3

Coomassie stained gel

GFP-SidJ

GFP

Calmodulin

a)

c) e)

d)

b)

t=0s t=10s t=20s t=30s t=40s

t=50s t=60s t=70s t=80s t=90s

0 500 1000 1500 2000

25000

30000

35000

40000

45000

50000

Time [s]

Fluorescence (AU)

Buffer

SdeA

SdeA + SidJ

SdeA + SidJ 1-81 9

0

200

300

100

0 0.5h 1h 3h 6h

time after infection

intracellular

[Ca

2+

] (nM)

PDE domain

mART domain

Residues 855-877

Solvent exposed (855-860)

E860

Legionella (encircled)=Red Endoplasmic reticulum (ER)=Green ER-cepia=Red

Extended Data Fig. 2 | See next page for caption.