Gaoet al.,Science 363 , eaau8302 (2019) 18 January 2019 9of16
Fig. 6. Long-range tracing and stereotypy of neuron bundles in
Drosophila.(A) MIP view of DC3 olfactory projection neurons (PNs)
projecting from the antenna lobe of an adultDrosophilabrain and partially
traced here (Movie 7) to the calyx (CA) and lateral horn (LH). Scale bar,
10 mm. (Inset) (White box) Comparison of cross-sectional views of the
axon bundle by means of (left) confocal microscopy and (right) ExLLSM.
Scale bar, 1mm. (Inset) (Yellow box) A magnified view of DC3 PN boutons
in CA. Scale bar, 1mm. (B) Volume of each individual DC3 PN bouton in
CA and LH. (C) Overlaid MIP view of DC3 PNs from five adultDrosophila
brains (D1 to D5) near CA. Scale bar, 10mm. (D) Number of DC3 PN
boutons in CA for D1 to D5 shown in (C). (E) Volume of DC3 PN boutons in
CA for D1 to D5 shown in (C). (F) MIP view of individually traced PPM3
DANs in the right hemisphere of an adultDrosophilabrain (Movie 8),
innervating the fan-shaped body (FB) (green), ellipsoid body (EB)
(magenta), and noduli (NO) (green). The fine neurites arborizing FB, EB,
and NO are from both hemispheres of the brain. Scale bar, 10mm.
(G) MIP view of the identified cell types of PPM3 DANs (fig. S20).
Scale bar, 10mm.RESEARCH | RESEARCH ARTICLE
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