Biotin-conjugated peptide
Manufacturer’s validation information: https://www.3helix.com/wp-
content/uploads/2019/04/3Helix_CHP_user_guide_20190326.pdf
Dilution used here: 15uM per tissue section
Detected with:
Alexa Fluor 647 Streptavidin
Supplier: Life Technologies, #S-21374
Lot: 1990312
Manufacturer’s validation information: https://www.thermofisher.com/document-connect/document-
connect.html?url=https%3A%2F%2Fassets.thermofisher.com%2FTFS-
Assets%2FBID%2Fcertificate%2FCertificates-of-
Analysis%2FS21374%2520Lot%25201990312%2520CofA.pdf&title=MTk5MDMxMg==
Dilution used here: 1:200
Assay: Flow Cytometry
CD3e, CD11b, CD45R, Ly-6G, or Ter119 (eBioscience #88-7774-75, as a biotinylated panel). Extended
Data Figure 1a.
CD3e: Lot # E02344-1633
CD11b: Lot # 4283483
CD45R: Lot # 4291976
Ly6G: Lot # E03072-1632
Ter199: Lot # 4283598Supplier: eBioscience, #88-7774-75
Manufacturer’s validation information: https://assets.thermofisher.com/TFS-Assets/LSG/manuals/88-
7774.pdf
Dilution used here: 1:50 for CD3e, 1:100 for each of the other antibodies
Detected with:
Streptavidin-conjugated Brilliant Violet 421
Supplier: BD Biosciences, #563259
Lot: 6077614
Manufacturer’s validation information: http://www.bdbiosciences.com/ds/pm/tds/563259.pdf
Dilution used here: 1:200
Brilliant Violet 421™ anti-mouse CD11b - Extended Data Figure 3b-e.
Supplier: BioLegend, #101251
Monoclonal
Lot: B260574
Manufacturer’s validation information: https://www.biolegend.com/en-us/products/brilliant-violet-421-
anti-mouse-human-cd11b-antibody-7163
Dilution used here: 1:100
Visualized directly with conjugated fluorophore.
Brilliant Violet 510™ anti-mouse Ly6c - Extended Data Figure 3c-d
Supplier: BioLegend #128033
Monoclonal