Extended Data Fig. 8 | Characterization of GPR174–GFP transgenic mice.
a, Diagrams of the GPR174–GFP fusion (top) and GPR174–GFP BAC construct
(bottom) in the context of the G p r1 74 genomic locus, with the five exons
numbered in italics. b, Transwell migration of lipopolysaccharide-activated B
cells that were transduced with a control, GPR174-expressing or GPR174-GFP-
expressing vector in response to stroma-conditioned medium (stroma) or
10 ng ml−1 or 50 ng ml−1 recombinant CCL21. Data represent three biological
replicates from one of two independent experiments with similar results.
c, d, Histograms (c) and geometric MFI (geo.mean) (d) of GFP f luorescence
from naive and germinal-centre B cells in B6 (grey histograms) or GPR174–GFP
BAC (open histograms) transgenic mice. Each symbol in the MFI plot
represents one mouse, and data represent two independent experiments with
similar results. Two-way ANOVA with Bonferroni’s multiple comparisons (b) or
two-tailed unpaired Student’s t-test (d) was used for comparisons between
groups. ****P < 0.0001; ns, not significant.