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nature research | reporting summary
April 2018
Data
Policy information about availability of data
All manuscripts must include a data availability statement. This statement should provide the following information, where applicable:
- Accession codes, unique identifiers, or web links for publicly available datasets
- A list of figures that have associated raw data
- A description of any restrictions on data availability
The raw and processed RNA-seq, ChIP-seq and ATAC-seq data are deposited on GEO (GSE118765 and GSE128958):
https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE118765
https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE128958
The following secure token has been created to allow review of record:
GSE118765: ersrqismjhypxex
GSE128958: ynupygawfdefdcp
Field-specific reporting
Please select the best fit for your research. If you are not sure, read the appropriate sections before making your selection.
Life sciences Behavioural & social sciences Ecological, evolutionary & environmental sciences
For a reference copy of the document with all sections, see nature.com/authors/policies/ReportingSummary-flat.pdf
Life sciences study design
All studies must disclose on these points even when the disclosure is negative.
Sample size For high throughput sequencing experiments, two independent biological replicates were used where possible and to strengthen the
conclusions of the study. Sample sized for other assays (qPCR, ChIP-PCR, etc.) were chosen based on our prior experience and common
standards in the field for detecting statistically significant differences between conditions.
Data exclusions No data was excluded from the studies.
Replication All attempts at replication were successful. Biological replicates of each experiment is stated under each figure legend and all attempts were
successful. Moreover, findings were repeatedly reproduced throughout the study: RNA-seq with qRT-PCR in multiple cell lines; protein levels
with Western blot and immunostaining; cell response assays with different cell line models.
Randomization In animal experiments, mice were randomly assigned to each experiment group.
Blinding Investigators were blinded to cell groups and mouse groups when collecting results.
Reporting for specific materials, systems and methods
Materials & experimental systems
n/a Involved in the study
Unique biological materials
Antibodies
Eukaryotic cell lines
Palaeontology
Animals and other organisms
Human research participants
Methods
n/a Involved in the study
ChIP-seq
Flow cytometry
MRI-based neuroimaging
Unique biological materials
Policy information about availability of materials
Obtaining unique materials DNA plasmids: pLVX-Tight-Puro-KrasG12D, pLVX-IRES-Hyg-SMAD4 (Addgene #107128), SGEP (Addgene #111170), pLenti-HA-
Rreb1, pSpCas9(BB)-2A-GFP (PX458) (Addgene #48138), pSpCas9(BB)-2A-Puro (PX459) (Addgene #48139), pLVX-EF1α-IRES-