22 | LAB+LIFE SCIENTIST - Feb/Mar 2019 http://www.LabOnline.com.au | http://www.LifeScientist.com.au
what’s new
Live lysosomal cell imaging
Cayman Chemical’s Lysosomal Staining Kit (red Fluorescence) is a convenient tool for imag-
ing lysosomes in live cells. The kit utilises a red fluorescent dye that permeates the lysosomes
based on ph gradient. Once protonated, the dye is unable to leave the lysosome, resulting in
enhanced fluorescence.
The kit includes bafilomycin A 1 , which is an inhibitor of the vacuolar ATpase. Treatment with
bafilomycin A 1 results in decreased lysosomal fluorescence upon staining with the lysosomal
staining reagent.
Lysosomes are intracellular organelles that contain enzymes used for the hydrolysis of waste
materials and other cellular debris. The ph of the lysosome is ~4.5 to 4.8 and is optimal for
these hydrolytic enzymes. The
lysosomal ph gradient is main-
tained through vacuolar ATpases
which pump protons into the
lysosomes.
Sapphire Bioscience
http://www.sapphirebioscience.comProtein analysis instrument
The Stunner from unchained Labs can measure both protein size and concentration in a
single instrument. It is designed specifically for protein and biologics researchers.
using uV/Vis spectroscopy and DLS (dynamic light scattering), the product is able to
simultaneously measure protein concentration, hydrodynamic size and polydispersity, and to
detect aggregates. This saves the user time, helping them to narrow down optimal formula-
tions more quickly.
The product requires only a 2 μL sample, with no need for dilution or sample preparation.
The system can process single samples in as little as 1 min. For higher throughputs, the
concentration of an entire 96 well-plate can be completed in 12 min, or both concentration
and sizing in 1 h. To provide the user with a more comprehensive picture of how their for-
mulation behaves, it can also determine the effects of parameters such as storage, agitation
and changes to process chemistry.
The instrument measures the concentration of each sample in a series using real-time DLS,
ensuring correct results and letting the user know if their sample is prone to aggregation.
The user can also determine the stability of their protein formulation using a host of stand
methods or they can create their own custom aggregation index.
AXT Pty Ltd
http://www.axt.com.au