Nature 2020 01 30 Part.02

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nature research | reporting summary

October 2018

For a reference copy of the document with all sections, see nature.com/documents/nr-reporting-summary-flat.pdf

Life sciences study design

All studies must disclose on these points even when the disclosure is negative.

Sample size For image analysis sample size (n=1068534) was chosen to assure the possibility to reach highest resolution

Data exclusions After 2-D classification, images corresponding to false particles or to dammages particles were excluded

Replication Gel shift and pull-down experiments were performed in triplicates and all attempts were successfull

Randomization All particles in the raw images were selected and analysed to ensure a unbiaised initial particle population

Blinding Image collection was performed automatically without human supervision or selection

Reporting for specific materials, systems and methods

We require information from authors about some types of materials, experimental systems and methods used in many studies. Here, indicate whether each material,

system or method listed is relevant to your study. If you are not sure if a list item applies to your research, read the appropriate section before selecting a response.

Materials & experimental systems

n/a Involved in the study

Antibodies

Eukaryotic cell lines

Palaeontology

Animals and other organisms

Human research participants

Clinical data

Methods

n/a Involved in the study

ChIP-seq

Flow cytometry

MRI-based neuroimaging

Eukaryotic cell lines

Policy information about cell lines

Cell line source(s) Pichia Pastoris X33 editied to add a SBP affinity tag on the endogenous Sgf73 subunit

Authentication sequencing

Mycoplasma contamination Light microscopy control showed no contaminating organism

Commonly misidentified lines

(See ICLAC register)

No commonly misidentified cell lines were used