and enzyme evolution. A.R.-G, E.C.S., D.V., and L.X. carried out
computational work to support enzyme discovery and evolution.
Competing interests:Merck & Co., Inc. has filed a patent
application covering the process: U.S. patent application no.
PCT/US2019/040316. Codexis, Inc. has filed patent applications
covering the evolved enzymes: U.S. patent application nos. PCT/
US2019/040353, PCT/US2019/040359, PCT/US2019/040369,
PCT/US2019/040376, and PCT/US2019/040379.Data and
materials availability:All data are available in the main text or the
supplementary materials.SUPPLEMENTARY MATERIALS
science.sciencemag.org/content/366/6470/1255/suppl/DC1
Materials and Methods
Figs. S1 to S28
Tables S1 to S15Schemes S1 to S81
DNA and amino acid sequences of the enzymes
NMR Spectra
HR-MS Spectra
References ( 30 – 50 )12 August 2019; accepted 24 October 2019
10.1126/science.aay8484Huffmanet al.,Science 366 , 1255–1259 (2019) 6 December 2019 5of5
RESEARCH | REPORT
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