The Scientist - USA (2020-05)

(Antfer) #1

COMINGSOON


Leveraging Advances in Predesigned Synthetic sgRNAs for
Highly Functional and Specific CRISPR-Cas9 Gene Knockout

WEBINAR SPONSORED BY

Each individual tumor is unique, carrying its own distinguishing antigens, which can complicate the cancer vaccine development process. Cancer vaccines
target unique tumor antigens, including overexpressed healthy proteins or virus-derived proteins. To better characterize, identify, and understand the
dynamic interactions between the immune system and cancer cell antigens, researchers can turn to advanced flow cytometry and live-cell imaging
technologies. This webinar, brought to you by The Scientist and sponsored by Sartorius, will present new research and developments in the cancer vaccine
field and delve into the intricacies of target discovery and vaccine development.

CRISPR-based genome editing has accelerated biological research and introduced great potential for studying and treating human diseases. The
CRISPR-Cas9 system requires a Cas9 nuclease and a guide RNA, which may consist of either a CRISPR RNA (crRNA) coupled with a trans-activating
crRNA (tracrRNA), or a single guide RNA (sgRNA) that combines the crRNA and tracrRNA in a single molecule. Both guide RNA formats can be
chemically synthesized, offering advantages over expression systems. This webinar, sponsored by Horizon Discovery, will explain how synthetic guide
RNAs are amenable to chemical modifications for increased stability, eliminate time-consuming steps of cloning and sequencing, and do not evoke
the inherent immune response and cytotoxicity that accompanies in vitro transcribed guide RNAs. They can be readily delivered into cells for high-
throughput arrayed screening applications to expand the types of phenotypic readouts to high-content and morphology-based assays.

DAVID E. AVIGAN, MD
Professor of Medicine
Beth Israel Deaconess Medical Center

KURT MARSHALL, PhD
R&D Scientist
Horizon Discovery

MARY L. (NORA) DISIS, MD
Helen B. Slonaker Endowed Professor
for Cancer Research
American Cancer Society Clinical
Research Professor
Professor, Medicine, Adjunct Professor Pathology
and Obstetrics and Gynecology,
University of Washington
Director, UW Medicine Cancer Vaccine Institute

THURSDAY, MA Y 21, 2020
2:30 - 4:00 PM EST

REGISTER NOW!
http://www.the-scientist.com/cancer-vaccines

REGISTER NOW!
http://www.the-scientist.com/synthetic-sgrna-crispr-
cas-knockout-horizon-discovery

TOPICS TO BE COVERED


  • Developing breast cancer vaccines with dendritic
    cell tumor fusions

  • Boosting immunity with cancer vaccines: A focus
    on breast and ovarian cancer immunology


TOPICS TO BE COVERED


  • How to use Edit-R Synthetic sgRNA for robust CRISPR
    gene knockout

  • Functionality of sgRNA in different cell models, including
    primary T cells

  • Simplifying high-throughput loss-of-function screening
    with sgRNAs


COMINGSOON Advancing Cancer Vaccines


TUESDAY, MA Y 12, 2020
2:30 - 4:00 PM EST

WEBINAR SPONSORED BY
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