Nature - USA (2019-07-18)

reSeArCH Letter

0 1234567

0.80

0.85

0.90

0.95

1.00

0123456701234567

0 1234567

0.80

0.85

0.90

0.95

1.00

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Relative body weight

Days post 5-FU

100 mg/kg150 mg/kg200 mg/kg

Young

Old

a

-7 -6 -5 -4 -3 -2 -1 012345

0.95

1.00

days

p.i.

Relative body weigh

t

Y ABC99 O ABC99

0.047

0.028

0.032

8x ip. ABC99

1 week 5-FU5 days Analysis

b

Extended Data Fig. 9 | Old intestine recovers poorly from 5-FU-
induced damage. a, Body weights of young and old mice following single
injection of 5-FU (100–200 mg kg−^1 ). Two mice per group, body weight
relative to day of injection (day 0). b, Relative body weight of young and
old mice treated for one week with or without ABC99 followed by single
5-FU (100 mg kg−^1 ) injection (n = 8 mice for young vehicle, old vehicle

and young ABC99-treated, n = 10 mice for old ABC99-treated). Daily data

points represent median (circles) and interquartile range (dashed line).

Daily weight of old ABC99-treated mice were compared to old controls

with two-tailed unpaired Student’s t-test; exact P values shown under the

corresponding daily weight. Young mice between 3 and 4 months of age;

old mice over 20 months of age.