Letter reSeArCH
c
YTH YTHDF2
YTH YTHDF2
Buffer edge Buffer edge
1 μg100 ng10 ng1 ng
0 m^6 A RNA
10 m^6 A RNA
1 m^6 A RNA Methylene blue
m6A immunoblot
0 m^6 A RNA
10 m^6 A RNA
1 m^6 A RNA
100 nts
0 m
6 A RNA
10 m
6 A RNA
1 m
6 A RNA
f
YTHDF2 Alexa488-YTHDF2
b
Alexa594-YTHDF1 Alexa647-YTHDF3DF3-Alexa64^7
d
Alexa594-YTHDF1 Alexa488-YTHDF2 Alexa647-YTHDF3 Merge
e
a
DF1
YTH (389 - 523)
DF2
YTH (410 - 544)
DF3
YTH (416 - 550)
Prion-like Background
Full-length protein
(^15) DF1
10
5
DF2 DF3
Prion-like domains
(^30) DF1
20
10
DF2 DF3
Amino acid composition (by percent)
Amino acids
1 0 1 0 1 0 g
Low complexity region (1-388)
Low complexity region (1-409)
Low complexity region (1-415)
domain composition *P-Xn-G motif
h
DF1DF2 DF3
0
1
2
DF2 Partition coefficient (A.U.)
BODIPY 10 m^6 A RNA
RNA
only
RNA
- DF2
0.8
1.0
1.2
1.4
1.6
Partition coefficient (A.U.)
(p < 0.0001)
Extended Data Fig. 1 | See next page for caption.