Nature - USA (2019-07-18)

Letter reSeArCH

c

YTH YTHDF2

YTH YTHDF2

Buffer edge Buffer edge

1 μg100 ng10 ng1 ng

0 m^6 A RNA

10 m^6 A RNA

1 m^6 A RNA Methylene blue

m6A immunoblot

0 m^6 A RNA

10 m^6 A RNA

1 m^6 A RNA

100 nts

0 m

6 A RNA

10 m

6 A RNA

1 m

6 A RNA

f

YTHDF2 Alexa488-YTHDF2

b

Alexa594-YTHDF1 Alexa647-YTHDF3DF3-Alexa64^7

d

Alexa594-YTHDF1 Alexa488-YTHDF2 Alexa647-YTHDF3 Merge

e

a

DF1

YTH (389 - 523)

DF2

YTH (410 - 544)

DF3

YTH (416 - 550)

Prion-like Background

Full-length protein

(^15) DF1
10
5
DF2 DF3
Prion-like domains
(^30) DF1
20
10
DF2 DF3
Amino acid composition (by percent)
Amino acids
1 0 1 0 1 0 g
Low complexity region (1-388)
Low complexity region (1-409)
Low complexity region (1-415)

domain composition *P-Xn-G motif

h
DF1DF2 DF3
0
1
2
DF2 Partition coefficient (A.U.)
BODIPY 10 m^6 A RNA

RNA
only
RNA

• DF2
  0.8
  1.0
  1.2
  1.4
  1.6
  Partition coefficient (A.U.)
  (p < 0.0001)
  Extended Data Fig. 1 | See next page for caption.