Nature - USA (2019-07-18)

reSeArCH Letter

Extended data table 1 | data collection and refinement statistics

a, Crystallographic data

Mgm1, SeMet

pdb code 6QL4

Data collection

Space group P4 1 22, 1 dimer/ASU

Cell dimensions

a, b, c (Å) 147.4, 147.4, 344.7

α, β, γ(°) 90, 90, 90

Wavelength 0.9794 Å

Resolution (Å)* 3.60 (3.60-3.69)

Rsym* (%) 22.0 (184)

I /σI* 8.0 (1.1)

Completeness (%)* 99.8 (98.4)

Redundancy 6.7

Refinement

Resolution (Å) 49.1 – 3.6

No. reflections 44,814

Rwork / Rfree 24.4 / 25.2

No. atoms

Protein 10,332

Ligand/ion 2

Water 0

B-factors

Protein 212 Å

2

Ligand/ion 101 Å

2

Water -

R.m.s deviations

Bond lengths (Å) 0.004

Bond angles (°) 0.986

b, Cryo-ET data

Mgm1+GTPγS

Outside

(EMDB-10063)

Apo Mgm1

Outside

(EMDB-10062)

Apo Mgm1

Outside,

overall tube

(EMDB-4584)

Mgm1+GTPγS

Inside

(EMDB-10065)

Apo Mgm1

Inside

(EMDB-10064)

Data collection

and processing

Magnification 53,000 53,000 53,000 53,000 53,000

Voltage (kV) 300 300 300 300 300

Electron exposure (e

• /Å
  2
  )
  Per tomogram

90 – 100 90 – 100 90 – 100 90 – 100 90 – 100

Defocus range (μm) 2 - 4 2 - 4 2 - 4 2 - 4 2 - 4

Pixel size (Å) 2.7 2.7 2.7 2.7 2.7

Symmetry imposed No No No No No

Initial particle images (no.) 71,884 12,440 2,214 4,751 1,874

Final particle images (no.) 9,471 11,474 1,677 1,820 1,792

Map resolution (Å)

FSC threshold

14.7

0.143

14.7

0.143

20.4

0.143

18.8

0.143

20.6

0.143

a, Data collection and refinement statistics for the crystallographic data.
b, Data collection and processing statistics for cryo-ET data.
*Values in parentheses are for the highest-resolution shell. The data were collected from a single crystal.